Research Article
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Published Online: 7 July 2004

Monoclonal Antibodies Against Human Telomerase Reverse Transcriptase (hTERT): Preparation, Characterization, and Application

Publication: Hybridoma
Volume 20, Issue Number 4

Abstract

The catalytic subunit of human telomerase reverse transcriptase (hTERT), is the necessary and rate-limiting component to telomerase activation in cancer cells. To develop monoclonal antibodies (MAbs) against hTERT, a peptide-hTERT9-derived from specific motif T of hTERT was synthesized. Through fusion of splenocytes of BALB/c mice immunized with hTERT9 with mouse myeloma cells, hybridomas were generated and clones secreting anti-hTERT9 antibody were screened. After three rounds of limited dilution of candidate clones, three of which present stable and constant antibody production. The MAbs were hTERT9-specific and reactive with native hTERT of human cancer cells or tissues in Western blot and immunohistochemistry. The heavy chain variable regions from three hybridomas were cloned and sequenced confirming their mouse Ig derivation. The described investigation suggested that the generated MAbs to hTERT9 could recognize native hTERT and be useful to cancer research.

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Published In

cover image Hybridoma
Hybridoma
Volume 20Issue Number 4August 2001
Pages: 249 - 255
PubMed: 11604111

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Published online: 7 July 2004
Published in print: August 2001

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Shaomin Yang
Department of Pathology, The Health Science Center of Peking University, Beijing 100083, China.
Bo Zhang
Department of Pathology, The Health Science Center of Peking University, Beijing 100083, China.
Junmei Wang
Department of Pathology, The Health Science Center of Peking University, Beijing 100083, China.
Songlin Liao
Department of Pathology, The Health Science Center of Peking University, Beijing 100083, China.
Jisheng Han
Department of Pathology, The Health Science Center of Peking University, Beijing 100083, China.
Jianping Wei
Department of Pathology, The Health Science Center of Peking University, Beijing 100083, China.
Lin Hou
Department of Pathology, The Health Science Center of Peking University, Beijing 100083, China.

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