Research Article
No access
Published Online: 7 July 2004

Comparative Assessment Expression of the Inhibitor of Growth 1 Gene (ING1) in Normal and Neoplastic Tissues

Publication: Hybridoma and Hybridomics
Volume 21, Issue Number 1

Abstract

Studies have indicated that the tumor suppressor p33ING1b (13q33-34) interact with p53. Moreover, the association of functional protein forms of each member of the p33ING1b/p53 complex is essential for optimum activity of p53. The present report describes the sequencing of cDNAs corresponding to the p33ING1b mRNAs in a series of normal and tumor cell lines, and the production of monoclonal antibodies (MAbs) reactive with p33ING1b. These antibodies were subsequently used to analyze p33ING1b expression in normal and tumor cell lines and tissues. No evidence of mutation of p33ING1b was found in any of the 15 tumor cell lines cDNAs studied. Our investigation of a wide range of normal tissues have shown that expression of the nuclear epitope is highly ubiquitous, whereas expression of the cytoplasmic form could be detected in only 50% of tissues studied. Considering neoplastic tissues, loss of nuclear p33ING1b was observed in melanoma, seminoma, papillary thyroid carcinoma, ductal breast carcinoma, and acute lymphoblastic leukemia. As with normal tissue, cytoplasmic p33ING1b was more restricted, being observed in around 30% of neoplastic tissues, but in melanoma, papillary thyroid carcinoma, ductal breast carcinoma, there was increased detection of cytoplasmic p33ING1b associated with concomitant loss of nuclear expression. These results may suggest that at least in some tumors, loss of effective p33ING1b function may be achieved by translocation to the cytoplasm or failure of nuclear localization.

Get full access to this article

View all available purchase options and get full access to this article.

Information & Authors

Information

Published In

cover image Hybridoma and Hybridomics
Hybridoma and Hybridomics
Volume 21Issue Number 1February 2002
Pages: 1 - 10
PubMed: 11991811

History

Published online: 7 July 2004
Published in print: February 2002

Permissions

Request permissions for this article.

Topics

Authors

Affiliations

Ghassan S. Nouman
Department of Pathology, Royal Victoria Infirmary, Newcastle upon Tyne, NE1 4PH, UK
Brian Angus
Department of Pathology, Royal Victoria Infirmary, Newcastle upon Tyne, NE1 4PH, UK
John Lunec
The Cancer Research Unit, The University of Newcastle-Upon-Tyne, Newcastle upon Tyne, NE1 4PH, UK
Steve Crosier
Department of Pathology, Royal Victoria Infirmary, Newcastle upon Tyne, NE1 4PH, UK
Andrew Lodge
Department of Pathology, Royal Victoria Infirmary, Newcastle upon Tyne, NE1 4PH, UK
John J. Anderson
Department of Pathology, Royal Victoria Infirmary, Newcastle upon Tyne, NE1 4PH, UK

Metrics & Citations

Metrics

Citations

Export citation

Select the format you want to export the citations of this publication.

View Options

Get Access

Access content

To read the fulltext, please use one of the options below to sign in or purchase access.

Society Access

If you are a member of a society that has access to this content please log in via your society website and then return to this publication.

Restore your content access

Enter your email address to restore your content access:

Note: This functionality works only for purchases done as a guest. If you already have an account, log in to access the content to which you are entitled.

View options

PDF/EPUB

View PDF/ePub

Media

Figures

Other

Tables

Share

Share

Copy the content Link

Share on social media

Back to Top