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Published Online: 7 July 2004

Monoclonal Antibodies Selective for Low Molecular Weight Neurofilaments

Publication: Hybridoma and Hybridomics
Volume 21, Issue Number 1


Neurofilaments are necessary for the maintenance of axonal caliber and structural organization of nerve cells. The low molecular weight form of neurofilament, the neurofilament light protein, which serves as the core of the filament, was used as immunogen for generation of hybridomas with selective reactivity with this form of the filament. Six hybridomas, out of approximately 100 tested clones, were highly discriminatory. All involved epitopes were localized to the rod region of the antigen, as determined by α-chymotrypsin digestion of the purified filament and enzymatic peptide mapping. Synthetic peptides (20 mers) covering the entire rod region did not react with the antibodies. A phage display peptide library was used to identify four consensus sequences for the antibodies. The results indicate that all epitopes were of conformational type. Pair-wise BIAcore data furthermore indicated that the epitopes were independent. The access to such specific reagents is a prerequisite for further elucidation of the biology of the low molecular weight form of neurofilaments proteins.

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cover image Hybridoma and Hybridomics
Hybridoma and Hybridomics
Volume 21Issue Number 1February 2002
Pages: 53 - 59
PubMed: 11991817


Published online: 7 July 2004
Published in print: February 2002


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Niklas Norgren
Department of Immunology, University of Umeå, Sweden
Jan-Erik Karlsson
Institute of Clinical Neuroscience, Department of Neurology, University of Göteborg, Sahlgrenska Hospital, Sweden
Lars Rosengren
Institute of Clinical Neuroscience, Department of Neurology, University of Göteborg, Sahlgrenska Hospital, Sweden
Torgny Stigbrand
Department of Immunology, University of Umeå, Sweden

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