Research Article
No access
Published Online: 7 July 2004

Monoclonal Antibodies Selective for Low Molecular Weight Neurofilaments

Publication: Hybridoma and Hybridomics
Volume 21, Issue Number 1

Abstract

Neurofilaments are necessary for the maintenance of axonal caliber and structural organization of nerve cells. The low molecular weight form of neurofilament, the neurofilament light protein, which serves as the core of the filament, was used as immunogen for generation of hybridomas with selective reactivity with this form of the filament. Six hybridomas, out of approximately 100 tested clones, were highly discriminatory. All involved epitopes were localized to the rod region of the antigen, as determined by α-chymotrypsin digestion of the purified filament and enzymatic peptide mapping. Synthetic peptides (20 mers) covering the entire rod region did not react with the antibodies. A phage display peptide library was used to identify four consensus sequences for the antibodies. The results indicate that all epitopes were of conformational type. Pair-wise BIAcore data furthermore indicated that the epitopes were independent. The access to such specific reagents is a prerequisite for further elucidation of the biology of the low molecular weight form of neurofilaments proteins.

Get full access to this article

View all available purchase options and get full access to this article.

Information & Authors

Information

Published In

cover image Hybridoma and Hybridomics
Hybridoma and Hybridomics
Volume 21Issue Number 1February 2002
Pages: 53 - 59
PubMed: 11991817

History

Published online: 7 July 2004
Published in print: February 2002

Permissions

Request permissions for this article.

Topics

Authors

Affiliations

Niklas Norgren
Department of Immunology, University of Umeå, Sweden
Jan-Erik Karlsson
Institute of Clinical Neuroscience, Department of Neurology, University of Göteborg, Sahlgrenska Hospital, Sweden
Lars Rosengren
Institute of Clinical Neuroscience, Department of Neurology, University of Göteborg, Sahlgrenska Hospital, Sweden
Torgny Stigbrand
Department of Immunology, University of Umeå, Sweden

Metrics & Citations

Metrics

Citations

Export citation

Select the format you want to export the citations of this publication.

View Options

Get Access

Access content

To read the fulltext, please use one of the options below to sign in or purchase access.

Society Access

If you are a member of a society that has access to this content please log in via your society website and then return to this publication.

Restore your content access

Enter your email address to restore your content access:

Note: This functionality works only for purchases done as a guest. If you already have an account, log in to access the content to which you are entitled.

View options

PDF/EPUB

View PDF/ePub

Media

Figures

Other

Tables

Share

Share

Copy the content Link

Share on social media

Back to Top