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Published Online: 7 July 2004

BRCA2 Monoclonal Antibodies React with Differentiating Epithelium

Publication: Hybridoma and Hybridomics
Volume 21, Issue Number 4

Abstract

The BRCA2 gene has previously been suggested to play a role in proliferation and DNA repair. Germline mutations in the BRCA2 gene predispose individuals to early onset, hereditary breast cancer. To better understand the expression pattern and function of the BRCA2 gene product, we have developed immunological reagents specific for BRCA2. These reagents recognize full-length (384 kDa) recombinant human BRCA2 proteins in transfected cell lysates as well as multiple smaller recombinant BRCA2 polypeptides. Detection of native BRCA2 protein in most tissue types, including breast epithelium, requires sensitive techniques such as immunoprecipitation-Western blot analysis. However, we have demonstrated strong reactivity of our immunological reagents with differentiating epithelium, including epidermis, thymic epithelium, and squamous cell carcinoma. These data suggest that BRCA2 may play a role in processes associated with cellular differentiation, in addition to its previously suggested roles in proliferation and DNA repair.

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Published In

cover image Hybridoma and Hybridomics
Hybridoma and Hybridomics
Volume 21Issue Number 4August 2002
Pages: 261 - 269
PubMed: 12193279

History

Published online: 7 July 2004
Published in print: August 2002

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    Lisa K. Gilliam
    Department of Pathology, Duke University Medical Center, Durham, NC 27710
    Edward K. Lobenhofer
    Department of Pathology and Program of Cell and Molecular Biology, Duke University Medical Center, Durham, NC 27710
    Paula K. Greer
    Department of Pathology, Duke University Medical Center, Durham, NC 27710
    Richard M. Scearce
    Department of Medicine, Duke University Medical Center, Durham, NC 27710
    Frank D. Cirisano
    Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, NC 27710
    Jeffrey R. Marks
    Department of Pathology, Department of Surgery and Program of Cell and Molecular Biology, Duke University Medical Center, Durham, NC 27710
    Laura P. Hale
    Department of Pathology and Program of Cell and Molecular Biology, Duke University Medical Center, Durham, NC 27710

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