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Published Online: 15 May 2014

Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors

Publication: ASSAY and Drug Development Technologies
Volume 12, Issue Number 4


Three-dimensional (3D) cell culture systems have gained increasing interest in drug discovery and tissue engineering due to their evident advantages in providing more physiologically relevant information and more predictive data for in vivo tests. In this review, we discuss the characteristics of 3D cell culture systems in comparison to the two-dimensional (2D) monolayer culture, focusing on cell growth conditions, cell proliferation, population, and gene and protein expression profiles. The innovations and development in 3D culture systems for drug discovery over the past 5 years are also reviewed in the article, emphasizing the cellular response to different classes of anticancer drugs, focusing particularly on similarities and differences between 3D and 2D models across the field. The progression and advancement in the application of 3D cell cultures in cell-based biosensors is another focal point of this review.

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Published In

cover image ASSAY and Drug Development Technologies
ASSAY and Drug Development Technologies
Volume 12Issue Number 4May 2014
Pages: 207 - 218
PubMed: 24831787


Published online: 15 May 2014
Published in print: May 2014


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Rasheena Edmondson
Biomanufacturing Research Institute and Technology Enterprises (BRITE), and Department of Pharmaceutical Sciences, North Carolina Central University, Durham, North Carolina.
Jessica Jenkins Broglie
Biomanufacturing Research Institute and Technology Enterprises (BRITE), and Department of Pharmaceutical Sciences, North Carolina Central University, Durham, North Carolina.
Audrey F. Adcock
Biomanufacturing Research Institute and Technology Enterprises (BRITE), and Department of Pharmaceutical Sciences, North Carolina Central University, Durham, North Carolina.
Liju Yang
Biomanufacturing Research Institute and Technology Enterprises (BRITE), and Department of Pharmaceutical Sciences, North Carolina Central University, Durham, North Carolina.


Address correspondence to:Liju Yang, PhDDepartment of Pharmaceutical Sciences and Biomanufacturing Research Institute and Technology Enterprises (BRITE)North Carolina Central UniversityDurham, NC 27707E-mail: [email protected]

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No competing financial interests exist.

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