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Published Online: 20 September 2006

NAD(P)H Oxidase Activity of Nox4 in Chondrocytes Is Both Inducible and Involved in Collagenase Expression

Publication: Antioxidants & Redox Signaling
Volume 8, Issue Number 9-10

Abstract

Reactive oxygen species (ROS) are regulators of redox-sensitive cell signaling pathways. In osteoarthritis, human interleukin-1β is implicated in cartilage destruction through an ROS-dependent matrix metalloproteinase production. To determine the molecular source of ROS production in the human IL-1β (hIL-1β)-sensitive chondrocyte immortalized cell line C-20/A4, transfected cells were constructed that overexpress NAD(P)H oxidases. First, RT–PCR analysis showed that the C-20/A4 cell line expressed Nox2, Nox4, p22 phox , and p67 phox , but not p47 phox . It was found that ROS production by C-20/A4 chondrocytes does not depend on PMA and ionomycin activation. This indicates that Nox2 was not involved in the production of ROS. In C- 20/A4 cells that overexpress Nox4, hIL-1β stimulated ROS production three times more than the normal production of C-20/A4 cells. Moreover, there was a fourfold increase in the production of collagenase (MMP-1) by chondrocytes that overexpress Nox4. Interestingly, MMP-1 production in cells that overexpress Nox2 was not sensitive to hIL-1β. These data suggest that under hIL-1β stimulation, C-20/A4 chondrocytes produce MMP-1 through a Nox4-mediated, ROS-dependent pathway.

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cover image Antioxidants & Redox Signaling
Antioxidants & Redox Signaling
Volume 8Issue Number 9-102006
Pages: 1485 - 1496
PubMed: 16987005

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Published online: 20 September 2006
Published in print: 2006

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Laurent Grange
GREPI EA 2938 UJF, Lab Enzymology/DBPC, Universitary Hospital A. Michallon, Grenoble, Cedex, France.
Rheumatology Department, Universitary Hospital A. Michallon, Grenoble, Cedex, France.
Minh Vu Chuong Nguyen
GREPI EA 2938 UJF, Lab Enzymology/DBPC, Universitary Hospital A. Michallon, Grenoble, Cedex, France.
Bernard Lardy
GREPI EA 2938 UJF, Lab Enzymology/DBPC, Universitary Hospital A. Michallon, Grenoble, Cedex, France.
Madiha Derouazi
GREPI EA 2938 UJF, Lab Enzymology/DBPC, Universitary Hospital A. Michallon, Grenoble, Cedex, France.
Yannick Campion
GREPI EA 2938 UJF, Lab Enzymology/DBPC, Universitary Hospital A. Michallon, Grenoble, Cedex, France.
Candice Trocme
GREPI EA 2938 UJF, Lab Enzymology/DBPC, Universitary Hospital A. Michallon, Grenoble, Cedex, France.
Marie–Helene Paclet
GREPI EA 2938 UJF, Lab Enzymology/DBPC, Universitary Hospital A. Michallon, Grenoble, Cedex, France.
Philippe Gaudin
Rheumatology Department, Universitary Hospital A. Michallon, Grenoble, Cedex, France.
Francoise Morel
GREPI EA 2938 UJF, Lab Enzymology/DBPC, Universitary Hospital A. Michallon, Grenoble, Cedex, France.

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