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Published Online: 24 February 2009

3-Mercaptopyruvate Sulfurtransferase Produces Hydrogen Sulfide and Bound Sulfane Sulfur in the Brain

Publication: Antioxidants & Redox Signaling
Volume 11, Issue Number 4

Abstract

Hydrogen sulfide (H2S) is a synaptic modulator as well as a neuroprotectant. Currently, pyridoxal-5′-phosphate (PLP)-dependent cystathionine β-synthase (CBS) is thought to be the major H2S-producing enzyme in the brain. We recently found that brain homogenates of CBS-knockout mice, even in the absence of PLP, produce H2S at levels similar to those of wild-type mice, suggesting the presence of another H2S-producing enzyme. Here we show that 3-mercaptopyruvate sulfurtransferase (3MST) in combination with cysteine aminotransferase (CAT) produces H2S from cysteine. In addition, 3MST is localized to neurons, and the levels of bound sulfane sulfur, the precursor of H2S, are greatly increased in the cells expressing 3MST and CAT but not increased in cells expressing functionally defective mutant enzymes. These data present a new perspective on H2S production and storage in the brain. Antioxid. Redox Signal. 11, 703–714.

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Published In

cover image Antioxidants & Redox Signaling
Antioxidants & Redox Signaling
Volume 11Issue Number 4April 2009
Pages: 703 - 714
PubMed: 18855522

History

Published in print: April 2009
Published online: 24 February 2009
Published ahead of production: 14 October 2008
Accepted: 10 October 2008
Revision received: 8 October 2008
Received: 20 August 2008

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Norihiro Shibuya
National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Kiyose, Tokyo, Japan.
Makiko Tanaka
National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Kiyose, Tokyo, Japan.
Mikiharu Yoshida
National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Kiyose, Tokyo, Japan.
Yuki Ogasawara
Meiji Pharmaceutical University, Kiyose, Tokyo, Japan.
Tadayasu Togawa
Meiji Pharmaceutical University, Kiyose, Tokyo, Japan.
Kazuyuki Ishii
Meiji Pharmaceutical University, Kiyose, Tokyo, Japan.
Hideo Kimura
National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Kiyose, Tokyo, Japan.

Notes

Address reprint requests to:
Dr. Hideo Kimura
National Institute of Neuroscience
National Center of Neurology and Psychiatry
4-1-1 Ogawahigashi
Kodaira, Tokyo, 187-8502, Japan
E-mail: [email protected]

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