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Published Online: 7 February 2012

Comparative Analysis of Nuclear Transfer Embryo-Derived Mouse Embryonic Stem Cells. Part II: Gene Regulation

Publication: Cellular Reprogramming (Formerly "Cloning and Stem Cells")
Volume 14, Issue Number 1

Abstract

In a mouse model nuclear transfer embryo-derived embryonic stem cell lines (ntESCs) of various genetic backgrounds and donor cell types were compared with reference ESCs and analyzed comprehensively at molecular level as a second part of a larger study. Expression profiles of ntESCs established by different NT-methods (piezoelectric microinjection or zona-free) were indistinguishable. However, expression profiling analyses identified differentially regulated genes between reference ESCs and ntESCs from different genetic backgrounds. A number of pluripotency and stemness marker genes significantly differed at the mRNA level between the cell lines. However, cluster and lineage analyses revealed that such differences had no effect on cell differentiation and cell fate. Regardless of the donor cell type, gene expression profiles of ntESCs were more similar to each other than to their counterpart fertilized embryo-derived ESCs of the same genotype. Overall, the results indicated that expression profile differences may be related to the genotype rather than to technical variations.

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cover image Cellular Reprogramming
Cellular Reprogramming (Formerly "Cloning and Stem Cells")
Volume 14Issue Number 1February 2012
Pages: 68 - 78
PubMed: 22204593

History

Published online: 7 February 2012
Published in print: February 2012
Published ahead of print: 28 December 2011

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Julianna Kobolak
Genetic Reprogramming Group, Agricultural Biotechnology Center, Gödöllő, Hungary.
BioTalentum Ltd., Gödöllő, Hungary.
Marion Horsch
Helmholtz Center Munich, German Research Center for Environmental Health, Institute of Experimental Genetics, Neuherberg, Germany.
Sandra Geißler
Helmholtz Center Munich, German Research Center for Environmental Health, Institute of Experimental Genetics, Neuherberg, Germany.
Solomon Mamo
*
Genetic Reprogramming Group, Agricultural Biotechnology Center, Gödöllő, Hungary.
Johannes Beckers
Helmholtz Center Munich, German Research Center for Environmental Health, Institute of Experimental Genetics, Neuherberg, Germany.
Technical University Munich, Center of Life and Food Sciences, Chair of Experimental Genetics, Weihenstephan, Freising, Germany.
Andras Dinnyes
Genetic Reprogramming Group, Agricultural Biotechnology Center, Gödöllő, Hungary.
Molecular Animal Biotechnology Laboratory, Szent Istvan University; Gödöllő, Hungary.
Department of Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.

Notes

Address correspondence to:Andras DinnyesMolecular Animal Biotechnology LaboratorySzent Istvan Universitypater K. u 1.H-2100 Gödöllö,Hungary
E-mail: [email protected]

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The authors declare that they have no competing interests.

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