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Published Online: 13 April 2012

Interaction of MTHFR 1298C with ACE D Allele Augments the Risk of Diabetic Nephropathy in Western Iran

Publication: DNA and Cell Biology
Volume 31, Issue Number 4

Abstract

The aim of the current study was to examine the influence of interaction between polymorphisms of methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C with angiotensin converting enzyme insertion/deletion (ACE I/D) polymorphism on the risk of diabetic nephropathy (DN). In a case control study using polymerase chain reaction (PCR)- and PCR-restriction fragment length polymorphism (RFLP), the presence of three polymorphisms in 140 patients with type 2 diabetes mellitus (T2DM) with nephropathy including patients with micro- and macro-albuminuria and 72 patients with normoalbuminuria from Western Iran were investigated. In the presence of both MTHFR 677 T and ACE D alleles, there was a trend toward increased risk of DN 2.68-fold (p=0.054). The possession of both MTHFR 677 T and ACE D alleles increased the risk of macro-albuminuria four times (p=0.035). The concomitant presence of both MTHFR 1298 C and ACE D alleles increased the risk of macro-albuminuria 7.8-fold (p=0.012). In addition, the risk of progression from micro- to macro-albuminuria in the presence of both alleles tended to be increased (4.1-fold, p=0.09). Our study for the first time demonstrated a synergistic effect between ACE I/D with either MTHFR C677T or A1298C polymorphism on the increased risk of DN among patients with T2DM. We found that MTHFR 1298 C strongly interacts with the ACE D allele and augments the risk of DN in our population.

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cover image DNA and Cell Biology
DNA and Cell Biology
Volume 31Issue Number 4April 2012
Pages: 553 - 559
PubMed: 21942443

History

Published online: 13 April 2012
Published in print: April 2012
Published ahead of print: 23 September 2011
Accepted: 12 August 2011
Revision received: 12 August 2011
Received: 28 June 2011

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Zohreh Rahimi
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Ali Hasanvand
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Pharmacology, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Vahid Felehgari
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Pharmacology, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Notes

Address correspondence to:Zohreh Rahimi, Ph.D.Department of BiochemistryMedical SchoolKermanshah University of Medical SciencesDaneshgah Ave.Kermanshah 67148-69914Iran
E-mail: [email protected]; [email protected]

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No competing financial interests exist for all authors.

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