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Published Online: 1 May 2015

Adenosine Receptor Transcriptomic Profile in Cardiac Tissue of a Zucker Rat Model

Publication: DNA and Cell Biology
Volume 34, Issue Number 5


To evaluate the possible variations of adenosine receptor (AR) profile together with TNF-α and IL-6 mRNA in cardiac tissue of obese Zucker rats (OZR) during fasting conditions (fc) and during the induction of acute hyperglycemia (AH). OZR (O, n=21) and age-matched lean control rats (CO, n=18) were studied during fc (COfc, n=8; Ofc, n=13) and during the induction of AH (COAH, n=10; OAH, n=8). The histopathologic analysis performed on O and CO heart samples did not show abnormalities of myocardial structure. The AR transcriptomic profile was analyzed in O and CO by real-time polymerase chain reaction (PCR) and a significantly lower mRNA expression was observed for A2AR in O with respect to CO (p=0.047), while a significant upregulation was observed for A3R in O with respect to CO (p=0.002). No significant differences between O and CO were observed for TNF-α or IL-6. Correlations were found between glycemia and A1R (p=0.03) and A2BR (p=0.002); total cholesterol and A2BR (p=0.02) and A3R (p=0.0002), as well as between IL-6 and A1R (p=0.05) and TNF-α and A2AR (p<0.0001). The modulation of ARs in these settings could represent a promising approach to pharmacological treatment, which must be supported by diet restrictions and physical exercise.

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Published In

cover image DNA and Cell Biology
DNA and Cell Biology
Volume 34Issue Number 5May 2015
Pages: 333 - 341
PubMed: 25710208


Published in print: May 2015
Published online: 1 May 2015
Published ahead of print: 24 February 2015
Accepted: 12 January 2015
Revision received: 22 December 2014
Received: 28 November 2014


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Manuela Cabiati
CNR Institute of Clinical Physiology, Biochemical and Molecular Biology Laboratory, Laboratory of Cardiovascular Biochemistry, Pisa, Italy.
Benedetta Svezia
CNR Institute of Clinical Physiology, Biochemical and Molecular Biology Laboratory, Laboratory of Cardiovascular Biochemistry, Pisa, Italy.
Maria Angela Guzzardi
CNR Institute of Clinical Physiology, Biochemical and Molecular Biology Laboratory, Laboratory of Cardiovascular Biochemistry, Pisa, Italy.
Letizia Mattii
Department of Clinical and Experimental Medicine, Unit of Histology and Medical Embryology, University of Pisa, Pisa, Italy.
Andrea D'Amico
Institute of Life Sciences, Scuola Superiore Sant'Anna, Pisa, Italy.
Chiara Caselli
CNR Institute of Clinical Physiology, Biochemical and Molecular Biology Laboratory, Laboratory of Cardiovascular Biochemistry, Pisa, Italy.
Tommaso Prescimone
CNR Institute of Clinical Physiology, Biochemical and Molecular Biology Laboratory, Laboratory of Cardiovascular Biochemistry, Pisa, Italy.
Maria Aurora Morales
CNR Institute of Clinical Physiology, Biochemical and Molecular Biology Laboratory, Laboratory of Cardiovascular Biochemistry, Pisa, Italy.
Silvia Del Ry
CNR Institute of Clinical Physiology, Biochemical and Molecular Biology Laboratory, Laboratory of Cardiovascular Biochemistry, Pisa, Italy.


Address correspondence to:Silvia Del Ry, ScDCNR Institute of Clinical PhysiologyBiochemical and Molecular Biology LaboratoryLaboratory of Cardiovascular BiochemistryVia Giuseppe Moruzzi 1Pisa 56124Italy
E-mail: [email protected]

Disclosure Statement

No competing financial interests exist.

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