The Shape of Vesicle-Containing Organelles Is Critical for Their Functions in Vesicle Endocytosis
Abstract
Exosomes are small vesicles secreted by a variety of cell types under physiological and pathological conditions. When Saccharomyces cerevisiae are grown in low glucose, small vesicles carrying more than 300 proteins with diverse biological functions are secreted. Upon glucose addition, secreted vesicles are endocytosed that requires cup-shaped organelles containing the major eisosome protein Pil1p at the rims. We aim to identify genes that regulate the function of cup-shaped organelles in vesicle endocytosis. In cells lacking either VID27 or VID21, Pil1p distribution was altered and cup-shaped organelles became elongated with narrower openings. Change in shape reduced the number of vesicles in the deeper areas and impaired vesicle endocytosis. Vid21p and Vid27p were localized to vesicle clusters and interacted with other Vid proteins. In the absence of these genes, these vesicles failed to aggregate and were secreted. Vid21p and Vid27p are required for the aggregation and retention of vesicles that contain Vid proteins in the cytoplasm. Increased vesicles near the plasma membrane in mutant strains correlate with an increased Pil1p movement resulting in the fusion of cup-shaped organelles. We conclude that the shape of vesicle-containing organelles is critical for their functions in vesicle endocytosis.
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Copyright 2017, Mary Ann Liebert, Inc.
History
Published in print: November 2017
Published online: 1 November 2017
Published ahead of print: 17 October 2017
Accepted: 19 August 2017
Revision received: 11 August 2017
Received: 7 July 2017
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Authors' Contributions
C.M.W. performed and analyzed the experiments shown in this article. L.Q.H.B. analyzed data and wrote the article. H.-L.C. contributed to the writing and preparation of figures for the article. All authors approved the final version of the article.
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These authors declare that they have no conflicts of interest with the contents of this article.
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