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Published Online: 12 September 2005

Prevalence of Antimicrobial Resistance Genes in Listeria monocytogenes Isolated from Dairy Farms

Publication: Foodbourne Pathogens & Disease
Volume 2, Issue Number 3

Abstract

Antimicrobial resistance of Listeria monocytogenes (n = 38) isolated from the four dairy farms to 15 antimicrobial agents was evaluated. All 38 L. monocytogenes isolates from the four farms evaluated were resistant to more than one antimicrobial in different combinations. All L. monocytogenes isolates evaluated were resistant to cephalosporin C (minimum inhibitory concentration [MIC] ≥ 512 µg/mL), streptomycin (MIC ≥ 32) and trimethoprim (MIC ≥ 512). Most L. monocytogenes isolates were resistant to ampicillin (92%, MIC ≥ 2), rifampicin (84%, MIC ≥ 4), rifamycin (84%, MIC ≥ 4), and florfenicol (66%, MIC ≥ 32) and some were resistant to tetracycline (45%, MIC ≥ 16), penicillin G (40%, MIC ≥ 2) and chloramphenicol (32%, MIC ≥ 32). All L. monocytogenes isolates were susceptible to amoxicillin, erythromycin, gentamicin, kanamycin and vancomycin. Susceptibility of L. monocytogenes to the antimicrobials evaluated was quite consistent among the dairy farms evaluated. However, some variability in antimicrobial susceptibility among dairy farms was noted. Nineteen of 38 L. monocytogenes isolates contained more than one antimicrobial resistance gene sequence. A high frequency of floR (66%) was found in L. monocytogenes followed by penA (37%), strA (34%), tetA (32%), and sulI (16%). Other tetracycline resistance genes (tetB, tetC, tetD, tetE, and tetG) and other antimicrobial resistance genes (cmlA, strB, aadA, sulI, vanA, vanB, ampC, ermB, ereA, and ereB) were not found in any of the L. monocytogenes isolates from the four dairy farms. Results of the present study demonstrated that L. monocytogenes isolated from the dairy farm environment were resistant to many antimicrobials and contained one or more antimicrobial resistance genes.

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Published In

cover image Foodborne Pathogens and Disease
Foodbourne Pathogens & Disease
Volume 2Issue Number 3Fall 2005
Pages: 201 - 211
PubMed: 16156701

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Published online: 12 September 2005
Published in print: Fall 2005

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V. Srinivasan
Food Safety Center of Excellence, University of Tennessee, Knoxville, Tennessee.
H.M. Nam
Food Safety Center of Excellence, University of Tennessee, Knoxville, Tennessee.
L.T. Nguyen
Food Safety Center of Excellence, University of Tennessee, Knoxville, Tennessee.
B. Tamilselvam
Food Safety Center of Excellence, University of Tennessee, Knoxville, Tennessee.
S.E. Murinda
Food Safety Center of Excellence, University of Tennessee, Knoxville, Tennessee.
S.P. Oliver
Food Safety Center of Excellence, University of Tennessee, Knoxville, Tennessee.

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