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Published Online: 7 August 2012

Evaluation of Bacterial Resistance to Essential Oils and Antibiotics After Exposure to Oregano and Cinnamon Essential Oils

Publication: Foodborne Pathogens and Disease
Volume 9, Issue Number 8

Abstract

Essential oils (EOs) are excellent antimicrobial agents sometimes used in active food packaging. This work studies the susceptibility of 48 clinical isolates and 12 reference strains of Gram-negative bacilli to oregano essential oil, cinnamon essential oil, and combinations of both. Furthermore, the tendency of the clinical isolates to develop resistance to these EOs and to different antibiotics after sequential oregano or cinnamon exposure was studied. For this purpose, antibiotic susceptibility (through disk diffusion assays and minimum inhibitory concentration [MIC] determination) and oregano and cinnamon susceptibility (through MIC and minimum bactericidal concentration [MBC] determination) were compared after 50 passages in the presence or absence of subinhibitory concentrations of oregano and cinnamon essential oils. The results showed that all strains were susceptible to both EOs and their combination independently of the antibiotic resistance profile. In addition, neither synergistic nor antagonistic effects were observed between oregano and cinnamon essential oils at the concentrations tested. After the sequential exposure to both EOs, only Serratia marcescens, Morganella morganii, and Proteus mirabilis treated with oregano changed their antibiotic resistance profile and/or increased their resistance to this EO. However, the changes in antibiotic and oregano resistance were not related.

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Published In

cover image Foodborne Pathogens and Disease
Foodborne Pathogens and Disease
Volume 9Issue Number 8August 2012
Pages: 699 - 705
PubMed: 22827568

History

Published online: 7 August 2012
Published in print: August 2012
Published ahead of print: 24 July 2012

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    Raquel Becerril
    Departamento de Química Analítica, Instituto de Investigación en Ingeniería de Aragón (I3A), Grupo GUIA, Universidad de Zaragoza, Zaragoza, Spain.
    Cristina Nerín
    Departamento de Química Analítica, Instituto de Investigación en Ingeniería de Aragón (I3A), Grupo GUIA, Universidad de Zaragoza, Zaragoza, Spain.
    Rafael Gómez-Lus
    Departamento de Microbiología Medicina Preventiva y Salud Pública, Instituto de Investigación de Ciencias Ambientales (IUCA), Universidad de Zaragoza, Zaragoza, Spain.

    Notes

    Address correspondence to:C. Nerín, Ph.D.Departamento de Química AnalíticaInstituto de Investigación en Ingeniería de Aragón (I3A)Grupo GUIAUniversidad de ZaragozaMa de Luna 350018 Zaragoza,Spain
    E-mail: [email protected]

    Disclosure Statement

    No competing financial interests exist.

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