Research Article
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Published Online: 26 June 2009

Long-Term Kinetics of Cytokine Responses in Human Tears After Penetrating Keratoplasty

Publication: Journal of Interferon and Cytokine Research
Volume 29, Issue Number 7

Abstract

This was a kinetic study of inflammatory cytokine levels in postoperative tear samples from penetrating keratoplasty (PKP) patients with or without corneal rejection. In a prospective design, nonstimulated tears were collected from the affected eyes of 12 patients at regular intervals for 12–14 months following PKP. Nine patients retained clear grafts, whereas three suffered endothelial rejection of the corneal graft within 14 months. The concentrations of the cytokines IL-1β, IL-6, TNF-α, IL-8, IL-10, and IL-12p70 were measured via cytometric bead array technology. The postoperative concentrations of the cytokines in the tears varied among the patients, but exhibited similar alteration patterns in each eye tested. The concentrations of IL-6 and IL-8 were significantly higher (P = 0.009 and P = 0.01, respectively), whereas those of IL-10, TNF-α, and IL-12p70 were significantly lower (P = 0.008, P = 0.006, and P = 0.0009, respectively) in the tear samples from the patients with corneal rejection as compared with those with uncomplicated corneal grafts. The ratios IL-6/IL-10 and IL-8/IL-10 were significantly higher (P = 0.0231 and P = 0.015, respectively), and TNF-α/IL-10 was significantly lower (P = 0.045) throughout the examination period in the patients with endothelial rejection. The enhanced release of IL-6 and IL-8 into the tears of patients with corneal graft rejection concomitant with decreased concentrations of IL-10, TNF-α, and IL-12p70 may possibly serve as an indicator of the rejection process. However, due to the large variation in the cytokine concentrations, the observed changes in tear composition do not categorically predict the final graft outcome.

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cover image Journal of Interferon & Cytokine Research
Journal of Interferon and Cytokine Research
Volume 29Issue Number 7July 2009
Pages: 375 - 380
PubMed: 19450148

History

Published in print: July 2009
Published online: 26 June 2009
Published ahead of print: 18 May 2009

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Mariann Fodor
Department of Ophthalmology, Medical and Health Sciences Centre, Faculty of Medicine, University of Debrecen, Hungary.
Péter Gogolák
Department of Immunology, Medical and Health Sciences Centre, Faculty of Medicine, University of Debrecen, Hungary.
Éva Rajnavölgyi
Department of Immunology, Medical and Health Sciences Centre, Faculty of Medicine, University of Debrecen, Hungary.
András Berta
Department of Ophthalmology, Medical and Health Sciences Centre, Faculty of Medicine, University of Debrecen, Hungary.
László Kardos
Medical Statistician, Head of Infection Control Services, Kenézy Hospital, Debrecen, Hungary.
László Módis
Department of Ophthalmology, Medical and Health Sciences Centre, Faculty of Medicine, University of Debrecen, Hungary.
Andrea Facskó
Department of Ophthalmology, Medical and Health Sciences Centre, Faculty of Medicine, University of Debrecen, Hungary.

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