The Effects of Dodecyl Maltoside and Sodium Dodecyl Sulfate Surfactants on the Stability and Aggregation of Recombinant Interferon Beta-1b
Publication: Journal of Interferon & Cytokine Research
Volume 34, Issue Number 11
Abstract
Aggregation often occurs during manufacturing and storage of protein drugs. Detergents such as sodium dodecyl sulfate are commonly used to prevent aggregation but need to be eliminated before final formulation for safety reasons. We studied the ability of dodecylmaltoside (DDM), a nontoxic alkyl saccharide surfactant, to reduce aggregation and increase the stability of interferon beta-1b (IFN)-β-1b. An increase of 8°C in the Tm of IFN-β-1b was observed when 0.1% of DDM was present in the protein solution. The absorption of DDM on hydrophobic surfaces of IFN-β-1b enables the surface to become hydrophilic and non-ionic, and increases the stability of the protein. 0.1% DDM also results in a 62% increase in helical and a 25% decrease in β-sheet structures. 0.1% DDM not only suppresses aggregate formation but also improves IFN-β-1b solubilization. Furthermore, we have showed the protective effect of DDM on the anti-viral activity of IFN-β-1b in solution.
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Information & Authors
Information
Published In
Journal of Interferon & Cytokine Research
Volume 34 • Issue Number 11 • November 2014
Pages: 894 - 901
PubMed: 24956236
Copyright
Copyright 2014, Mary Ann Liebert, Inc.
History
Published online: 5 November 2014
Published in print: November 2014
Published ahead of print: 23 June 2014
Accepted: 29 April 2014
Received: 10 December 2013
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No competing financial interests exist.
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