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Published Online: 11 October 2011

Leaf Extract of Moringa oleifera Prevents Ionizing Radiation-Induced Oxidative Stress in Mice

Publication: Journal of Medicinal Food
Volume 14, Issue Number 10

Abstract

The present study evaluated the hepatoprotective effect of aqueous ethanolic Moringa oleifera leaf extract (MoLE) against radiation-induced oxidative stress, which is assessed in terms of inflammation and lipid peroxidation. Swiss albino mice were administered MoLE (300 mg/kg of body weight) for 15 consecutive days before exposing them to a single dose of 5 Gy of 60Co γ-irradiation. Mice were sacrificed at 4 hours after irradiation. Liver was collected for immunoblotting and biochemical tests for the detection of markers of hepatic oxidative stress. Nuclear translocation of nuclear factor kappa B (NF-κB) and lipid peroxidation were augmented, whereas the superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and ferric reducing antioxidant power (FRAP) values were decreased by radiation exposure. Translocation of NF-κB from cytoplasm to nucleus and lipid peroxidation were found to be inhibited, whereas increases in SOD, CAT, GSH, and FRAP were observed in the mice treated with MoLE prior to irradiation. Therefore pretreatment with MoLE protected against γ-radiation-induced liver damage. The protection may be attributed to the free radical scavenging activity of MoLE, through which it can ameliorate radiation-induced oxidative stress.

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References

1.
Makkar HPSBecker K. Nutritional value and antinutritional components of whole and ethanol extracted Moringa oleifera leavesAnim Feed Sci Technol199663211-228. 1. Makkar HPS, Becker K: Nutritional value and antinutritional components of whole and ethanol extracted Moringa oleifera leaves. Anim Feed Sci Technol 1996;63:211–228.
2.
Selvakumar DNatarajan P. Hepatoprotective activity of Moringa oleifera Lam in carbon tetrachloride induced hepatotoxicity in albino ratsPhcog Mag2008497-98. 2. Selvakumar D, Natarajan P: Hepatoprotective activity of Moringa oleifera Lam in carbon tetrachloride induced hepatotoxicity in albino rats. Phcog Mag 2008;4:97–98.
3.
Fakurazi SHairuszah INanthini U. Moringa oleifera Lam prevents acetaminophen induced liver injury through restoration of glutathione levelFood Chem Toxicol2008462611-2615. 3. Fakurazi S, Hairuszah I, Nanthini U: Moringa oleifera Lam prevents acetaminophen induced liver injury through restoration of glutathione level. Food Chem Toxicol 2008;46:2611–2615.
4.
Rao AVDevi PUKamarth R. In vivo radioprotective effect of Moringa oleifera leavesIndian J Exp Biol200139858-863. 4. Rao AV, Devi PU, Kamarth R: In vivo radioprotective effect of Moringa oleifera leaves. Indian J Exp Biol 2001;39:858–863.
5.
Oktay MCulcin IKufrevioglu OI. Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extractsLebensmittel Wissenschaft Technol200336263-271. 5. Oktay M, Culcin I, Kufrevioglu OI: Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. Lebensmittel Wissenschaft Technol 2003;36:263–271.
6.
Re RPellegrini NProteggente APannala AYang MRice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorizing assayFree Radic Biol Med1999261231-1237. 6. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C: Antioxidant activity applying an improved ABTS radical cation decolorizing assay. Free Radic Biol Med 1999;26:1231–1237.
7.
Lowry OHRosebrough NJFarr ALRandall RJ. Protein measurement with the Folin phenol reagentJ Biol Chem1951193265-275. 7. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265–275.
8.
Ghose ACMookerjee KSengupta AKGhosh AKDasgupta SRay PK. Therapeutic and prophylactic uses of protein A in the control of Leishmania donovani infection in experimental animalsImmunol Lett199965175-181. 8. Ghose AC, Mookerjee K, Sengupta AK, Ghosh AK, Dasgupta S, Ray PK: Therapeutic and prophylactic uses of protein A in the control of Leishmania donovani infection in experimental animals. Immunol Lett 1999;65:175–181.
9.
Beige AJAust SD. Microsomal lipid-peroxidationMethods Enzymol197852302-310. 9. Beige AJ, Aust SD: Microsomal lipid-peroxidation. Methods Enzymol 1978;52:302–310.
10.
Marklund SMarklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutaseEur J Biochem197447469-474. 10. Marklund S, Marklund G: Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974;47:469–474.
11.
Yumoto IIchihashi DIwata HIstokovics AIchise NMatsuyama HOkuyama HKawasaki K. Purification and characterization of a catalase from the facultatively psychrophilic bacterium Vibrio rumoiensis S-1T exhibiting high catalase activityJ Bacteriol20001821903-1909. 11. Yumoto I, Ichihashi D, Iwata H, Istokovics A, Ichise N, Matsuyama H, Okuyama H, Kawasaki K: Purification and characterization of a catalase from the facultatively psychrophilic bacterium Vibrio rumoiensis S-1T exhibiting high catalase activity. J Bacteriol 2000;182:1903–1909.
12.
Moron MSDepierre JWMannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lungs and liverBiochim Biophys Acta197958267-78. 12. Moron MS, Depierre JW, Mannervik B: Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lungs and liver. Biochim Biophys Acta 1979;582:67–78.
13.
Benzie IFFStrain JJ. Ferric reducing ability of plasma as a measure of “antioxidant power”: the FRAP assayAnal Biochem199623970-76. 13. Benzie IFF, Strain JJ: Ferric reducing ability of plasma as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 1996;239:70–76.
14.
Stajner DMilosevic MPopovic BM. Irradiation effects on phenolic content, lipid and protein oxidation and scavenger ability of soya bean seedInt J Mol Sci20078618-627. 14. Stajner D, Milosevic M, Popovic BM: Irradiation effects on phenolic content, lipid and protein oxidation and scavenger ability of soya bean seed. Int J Mol Sci 2007;8:618–627.
15.
Brach MAHass RSherman ML. Ionizing radiation induces expression and binding activity of nuclear factor kappa BJ Clin Invest199188691-695. 15. Brach MA, Hass R, Sherman ML: Ionizing radiation induces expression and binding activity of nuclear factor kappa B. J Clin Invest 1991;88:691–695.
16.
Li QVerma IM. NF-κB regulation in the immune systemNat Rev Immunol20022725-734. 16. Li Q, Verma IM: NF-κB regulation in the immune system. Nat Rev Immunol 2002;2:725–734.
17.
Jagetia GCReddy TK. Modulation of radiation-induced alteration in the antioxidant status of mice by naringinLife Sci200577780-794. 17. Jagetia GC, Reddy TK: Modulation of radiation-induced alteration in the antioxidant status of mice by naringin. Life Sci 2005;77:780–794.
18.
Greenberg MELi XMGugiu BGGu XQjn JSalomon RGHazen SL. The lipid whisker model of the structure of oxidized cell membranesJ Biol Chem20082832385-2396. 18. Greenberg ME, Li XM, Gugiu BG, Gu X, Qjn J, Salomon RG, Hazen SL: The lipid whisker model of the structure of oxidized cell membranes. J Biol Chem 2008;283:2385–2396.
19.
West JDMarnett LJ. Endogenous reactive intermediates as modulators of cell signaling and cell deathChem Res Toxicol200619173-194. 19. West JD, Marnett LJ: Endogenous reactive intermediates as modulators of cell signaling and cell death. Chem Res Toxicol 2006;19:173–194.
20.
Esterbauer HSchaur JSZollner H. Chemistry and biochemistry of 4-hydroxynonenal, alonaldehyde and related aldehydesFree Radic Biol Med19911181-128. 20. Esterbauer H, Schaur JS, Zollner H: Chemistry and biochemistry of 4-hydroxynonenal, alonaldehyde and related aldehydes. Free Radic Biol Med 1991;11:81–128.
21.
Poli GBiasi FLeonarduzzi G. 4-Hydroxynonenal-protein adducts: a reliable biomarker of of lipid oxidation in liver diseaseMol Aspects Med20082967-71. 21. Poli G, Biasi F, Leonarduzzi G: 4-Hydroxynonenal-protein adducts: a reliable biomarker of of lipid oxidation in liver disease. Mol Aspects Med 2008;29:67–71.
22.
Uchida K. 4-Hydroxy-2-nonenal: a product and modulator of oxidative stressProg Lipid Res200342318-343. 22. Uchida K: 4-Hydroxy-2-nonenal: a product and modulator of oxidative stress. Prog Lipid Res 2003;42:318–343.

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Published In

cover image Journal of Medicinal Food
Journal of Medicinal Food
Volume 14Issue Number 10October 2011
Pages: 1167 - 1172
PubMed: 21861723

History

Published online: 11 October 2011
Published in print: October 2011
Published ahead of print: 23 August 2011
Accepted: 29 May 2011
Received: 19 November 2010

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Mahuya Sinha
Department of Physiology, University Colleges of Science, Technology, and Agriculture, University of Calcutta, Kolkata, West Bengal, India.
Dipesh K. Das
Department of Physiology, University Colleges of Science, Technology, and Agriculture, University of Calcutta, Kolkata, West Bengal, India.
Surajit Bhattacharjee
Department of Molecular Medicine, Bose Institute, Kolkata, West Bengal, India.
Subrata Majumdar
Department of Molecular Medicine, Bose Institute, Kolkata, West Bengal, India.
Sanjit Dey
Department of Physiology, University Colleges of Science, Technology, and Agriculture, University of Calcutta, Kolkata, West Bengal, India.

Notes

Address correspondence to: Dr. Sanjit Dey, Department of Physiology, UCSTA, University of Calcutta, 92 APC Road, Kolkata 700009, West Bengal, India, E-mail: [email protected]

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No competing financial interests exist.

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