Research Article
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Published Online: 10 April 2014

Probiotic Screening and Safety Evaluation of Lactobacillus Strains from Plants, Artisanal Goat Cheese, Human Stools, and Breast Milk

Publication: Journal of Medicinal Food
Volume 17, Issue Number 4

Abstract

The aim of this study was to select autochthonous strains of Lactobacillus from stools of healthy infants and adults, human milk, artisanal goat cheese, and fruits and vegetables according to their probiotic properties and safety. From 421 strains of Lactobacillus isolated, 102 (24.2%) were shown to be tolerant to gastric pH and bile salts; they were used to determine their anti-Helicobacter pylori (agar diffusion assay), antioxidant (oxygen radical absorption capacity), and anti-inflammatory (inhibition of interleukin-8 release by tumor necrosis factor-α-stimulated HT-29 cells) activities as well as their ability to adhere to intestinal (Caco-2) and gastric (AGS) epithelial cells. Results obtained were compared with three commercial probiotic Lactobacillus rhamnosus GG, L. plantarum 299v, and L. johnsonii NCC533. The five strains most efficient according to these activities were subsequently identified by sequencing their 16S rRNA gene, their susceptibility to antibiotics was determined, and their safety evaluated in mice. One strain of L. plantarum was discarded due to the higher prevalence of liver bacterial translocation observed in the animals fed this strain. In conclusion, four autochthonous strains of L. rhamnosus were finally selected with probiotic properties and safety allowing their eventual use in human studies. These results contribute to increase the diversity of probiotic strains available for the development of nutraceuticals and functional foods.

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Information & Authors

Information

Published In

cover image Journal of Medicinal Food
Journal of Medicinal Food
Volume 17Issue Number 4April 2014
Pages: 487 - 495
PubMed: 24433075

History

Published online: 10 April 2014
Published in print: April 2014
Published ahead of print: 16 January 2014
Accepted: 7 October 2013
Received: 1 February 2012

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Authors

Affiliations

Martin Gotteland
Laboratories of Microbiology and Probiotics, University of Chile, Santiago, Chile.
Department of Nutrition, Faculty of Medicine, University of Chile, Santiago, Chile.
Maria Jose Cires
Laboratories of Microbiology and Probiotics, University of Chile, Santiago, Chile.
Department of Nutrition, Faculty of Medicine, University of Chile, Santiago, Chile.
Claudia Carvallo
Laboratories of Microbiology and Probiotics, University of Chile, Santiago, Chile.
Natalia Vega
Laboratories of Microbiology and Probiotics, University of Chile, Santiago, Chile.
Maria Antonieta Ramirez
Laboratories of Microbiology and Probiotics, University of Chile, Santiago, Chile.
Pamela Morales
Laboratories of Microbiology and Probiotics, University of Chile, Santiago, Chile.
Department of Nutrition, Faculty of Medicine, University of Chile, Santiago, Chile.
Patricia Rivas
Laboratories of Microbiology and Probiotics, University of Chile, Santiago, Chile.
Fernanda Astudillo
Laboratories of Microbiology and Probiotics, University of Chile, Santiago, Chile.
Paola Navarrete
Laboratories of Biotechnology, University of Chile, Santiago, Chile.
Céline Dubos
Laboratories of Microbiology and Probiotics, University of Chile, Santiago, Chile.
Alvaro Figueroa
Laboratories of Microbiology and Probiotics, University of Chile, Santiago, Chile.
Miriam Troncoso
Laboratories of Microbiology and Probiotics, University of Chile, Santiago, Chile.
Carolina Ulloa
Laboratories of Microbiology and Probiotics, University of Chile, Santiago, Chile.
Maria Luisa Mizgier
Laboratories of Microbiology and Probiotics, University of Chile, Santiago, Chile.
Catalina Carrasco-Pozo
Laboratories of Microbiology and Probiotics, University of Chile, Santiago, Chile.
Department of Nutrition, Faculty of Medicine, University of Chile, Santiago, Chile.
Hernan Speisky
Laboratories of Antioxidants, Institute of Nutrition and Food Technology (INTA), University of Chile, Santiago, Chile.
Oscar Brunser
Laboratories of Microbiology and Probiotics, University of Chile, Santiago, Chile.
Guillermo Figueroa
Laboratories of Microbiology and Probiotics, University of Chile, Santiago, Chile.

Notes

Address correspondence to: Martin Gotteland, PhD, Department of Nutrition, Faculty of Medicine, University of Chile, Independencia 1027, Independencia, Santiago 13811, Chile, E-mail: [email protected]

Author Disclosure Statement

No competing financial interests exist.

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