Probiotic Screening and Safety Evaluation of Lactobacillus Strains from Plants, Artisanal Goat Cheese, Human Stools, and Breast Milk
Publication: Journal of Medicinal Food
Volume 17, Issue Number 4
Abstract
The aim of this study was to select autochthonous strains of Lactobacillus from stools of healthy infants and adults, human milk, artisanal goat cheese, and fruits and vegetables according to their probiotic properties and safety. From 421 strains of Lactobacillus isolated, 102 (24.2%) were shown to be tolerant to gastric pH and bile salts; they were used to determine their anti-Helicobacter pylori (agar diffusion assay), antioxidant (oxygen radical absorption capacity), and anti-inflammatory (inhibition of interleukin-8 release by tumor necrosis factor-α-stimulated HT-29 cells) activities as well as their ability to adhere to intestinal (Caco-2) and gastric (AGS) epithelial cells. Results obtained were compared with three commercial probiotic Lactobacillus rhamnosus GG, L. plantarum 299v, and L. johnsonii NCC533. The five strains most efficient according to these activities were subsequently identified by sequencing their 16S rRNA gene, their susceptibility to antibiotics was determined, and their safety evaluated in mice. One strain of L. plantarum was discarded due to the higher prevalence of liver bacterial translocation observed in the animals fed this strain. In conclusion, four autochthonous strains of L. rhamnosus were finally selected with probiotic properties and safety allowing their eventual use in human studies. These results contribute to increase the diversity of probiotic strains available for the development of nutraceuticals and functional foods.
Get full access to this article
View all available purchase options and get full access to this article.
References
1.
Dunne C, O'Mahony L, Murphy L, et al.: In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. Am J Clin Nutr 2001;73:386S–392S.
2.
Dubos C, Vega N, Carvallo C, et al.: Identification of Lactobacillus spp. in colostrum from Chilean mothers. Arch Latinoam Nutr 2011;61:66–68.
3.
Food and Agriculture Organization of the United Nations (FAO): Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria. www.who.int/foodsafety/publications/fs_management/en/probiotics.pdf (accessed December 2013).
4.
Figueroa-González I, Quijano G, Ramírez G, Cruz-Guerrero A: Probiotics and prebiotics—perspectives and challenges. J Sci Food Agric 2011;91:1341–1348.
5.
Garrido D, Suau A, Pochart P, Cruchet S, Gotteland M: Modulation of the fecal microbiota by the intake of a Lactobacillus johnsonii La1-containing product in human volunteers. FEMS Microbiol Lett 2005;248:249–256.
6.
Brunser O, Figueroa G, Gotteland M, et al.: Effects of probiotic or prebiotic supplemented milk formulas on fecal microbiota composition of infants. Asia Pac J Clin Nutr 2006;15:368–376.
7.
Brunser O, Gotteland M, Cruchet S, Figueroa G, Garrido D, Steenhout P: Effect of a milk formula with prebiotics on the intestinal microbiota of infants after an antibiotic treatment. Pediatr Res 2006;59:451–456.
8.
Caceres P, Montes S, Vega N, Cruchet S, Brunser O, Gotteland M: Effects of Lactobacillus rhamnosus HN001 on acute respiratory infections and intestinal secretory IgA in children. J Pediatr Infect Dis 2010;5:353–362.
9.
Ko JS, Yang HR, Chang JY, Seo JK: Lactobacillus plantarum inhibits epithelial barrier dysfunction and interleukin-8 secretion induced by tumor necrosis factor-alpha. World J Gastroenterol 2007;13:1962–1965.
10.
Cao G, Alessio HM, Cutler RG: Oxygen-radical absorbance capacity assay for antioxidants. Free Radic Biol Med 1993;14:303–311.
11.
Zhou JS, Shu Q, Rutherfurd KJ, Prasad J, Gopal PK, Gil HS: Acute oral toxicity and bacterial translocation studies on potentially probiotic strains of lactic acid bacteria. Food Chem Toxicol 2000;38:153–161.
12.
Charteris WP, Kelly PM, Morelli L, Collins JK: Antibiotic susceptibility of potentially probiotic Lactobacillus species. J Food Prot 1998;61:1636–1643.
13.
Zhou JS, Pillidge CJ, Gopal PK, Gill HS: Antibiotic susceptibility profiles of new probiotic Lactobacillus and Bifidobacterium strains. Int J Food Microbiol 2005;98:211–217.
14.
Zhou JS, Shu Q, Rutherfurd KJ, et al.: Safety assessment of potential probiotic lactic acid bacterial strains Lactobacillus rhamnosus HN001, Lb. acidophilus HN017, and Bifidobacterium lactis HN019 in BALB/c mice. Int J Food Microbiol 2000;56:87–96.
15.
International Scientific Association for Probiotics and Prebiotics (ISAPP): Clarification of the Definition of a Probiotic. www.isapp.net/docs/ProbioticDefinition.pdf (accessed December 2013).
16.
Gueimonde M, Salminen S: New methods for selecting and evaluating probiotics. Dig Liver Dis 2006;38:S242–S247.
17.
Kirtzalidou E, Pramateftaki P, Kotsou M, Kyriacou A: Screening for lactobacilli with probiotic properties in the infant gut microbiota. Anaerobe 2011;17:440–443.
18.
Lee J, Yun HS, Cho KW, et al.: Evaluation of probiotic characteristics of newly isolated Lactobacillus spp.: immune modulation and longevity. Int J Food Microbiol 2011;148:80–86.
19.
Maragkoudakis PA, Zoumpopoulou G, Miaris C, Kalantzopoulos G, Pot B, Tsakalidou E: Probiotic potential of Lactobacillus strains isolated from dairy products. Int Dairy J 2006;16:189–199.
20.
Lee NK, Yun CW, Kim SW, Chang HI, Kang CW, Paik HD: Screening of lactobacilli derived from chicken feces and partial characterization of Lactobacillus acidophilus A12 as an animal probiotics. J Microbiol Biotechnol 2008;18:338–342.
21.
Vesterlund S, Paltta J, Karp M, Ouwehand AC: Measurement of bacterial adhesion-in vitro evaluation of different methods. J Microbiol Methods 2005;60:225–233.
22.
Apostolou E, Kirjavainen PV, Saxelin M, et al.: Good adhesion properties of probiotics: a potential risk for bacteremia? FEMS Immunol Med Microbiol 2001;31:35–39.
23.
Backert S, Clyne M: Pathogenesis of Helicobacter pylori infection. Helicobacter 2011;16:19–25.
24.
Gotteland M, Brunser O, Cruchet S: Systematic review: are probiotics useful in controlling gastric colonization by Helicobacter pylori? Aliment Pharmacol Ther 2006;23:1077–1086.
25.
Michetti P, Dorta G, Wiesel PH, et al.: Effect of whey-based culture supernatant of Lactobacillus acidophilus (johnsonii) La1 on Helicobacter pylori infection in humans. Digestion 1999;60:203–209.
26.
Martarelli D, Verdenelli MC, Scuri S, et al.: Effect of a probiotic intake on oxidant and antioxidant parameters in plasma of athletes during intense exercise training. Curr Microbiol 2011;62:1689–1696.
27.
Saide JA, Gilliland SE: Antioxidative activity of lactobacilli measured by oxygen radical absorbance capacity. J Dairy Sci 2005;88:1352–1357.
28.
Vizoso Pinto MG, Rodriguez Gómez M, Seifert S, Watzl B, Holzapfel WH, Franz CM: Lactobacilli stimulate the innate immune response and modulate the TLR expression of HT29 intestinal epithelial cells in vitro. Int J Food Microbiol 2009;133:86–93.
29.
Borriello SP, Hammes WP, Holzapfel W, et al.: Safety of probiotics that contain lactobacilli or bifidobacteria. Clin Infect Dis 2003;36:775–780.
30.
Cannon JP, Lee TA, Bolanos JT, Danziger LH: Pathogenic relevance of Lactobacillus: a retrospective review of over 200 cases. Eur J Clin Microbiol Infect Dis 2005;24:31–40.
31.
Salminen MK, Rautelin H, Tynkkynen S, et al.: Lactobacillus bacteremia, species identification, and antimicrobial susceptibility of 85 blood isolates. Clin Infect Dis 2006;42:35–44.
32.
Vankerckhoven V, Huys G, Vancanneyt M, et al.: Biosafety assessment of probiotics used for human consumption: recommendations from the EU-PROSAFE Project. Trends Food Sci Technol 2008;19:102–114.
33.
Hale P, Hill A: The recovery of Lactobacillus sp. from the livers of healthy mice. Lab Anim 1973;7:119–124.
Information & Authors
Information
Published In
Copyright
Copyright 2014, Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition.
History
Published online: 10 April 2014
Published in print: April 2014
Published ahead of print: 16 January 2014
Accepted: 7 October 2013
Received: 1 February 2012
Topics
Authors
Author Disclosure Statement
No competing financial interests exist.
Metrics & Citations
Metrics
Citations
Export Citation
Export citation
Select the format you want to export the citations of this publication.
View Options
Get Access
Access content
To read the fulltext, please use one of the options below to sign in or purchase access.⚠ Society Access
If you are a member of a society that has access to this content please log in via your society website and then return to this publication.