Effect of Intraperitoneal Administered Ginseng Total Saponins on Hyperalgesia Induced by Repeated Intramuscular Injection of Acidic Saline in Rats
Publication: Journal of Medicinal Food
Volume 17, Issue Number 6
Abstract
The aim of this study was to assess the antinociceptive activity of ginseng total saponins (GTS) on hyperalgesia induced by repeated intramuscular injections of acidic saline in rats and to examine the mechanisms involved. Rats were injected intraperitoneally with a 0.9% saline vehicle or various doses of GTS after the development of hyperalgesia. Rats were then injected with N-methyl-D-aspartate (NMDA) or naloxone 10 min before GTS injection. The mechanical withdrawal threshold (MWT) was assessed with von Frey filaments. The MWT was significantly increased after intraperitoneal injection of 100 mg/kg and 200 mg/kg of GTS when compared with the MWT after the development of hyperalgesia. Injection of GTS with NMDA showed a significant decrease in the MWT when compared with GTS injection. GTS showed an antinociceptive activity against chronic muscle-induced pain, and the effect of GTS may be mediated by NMDA.
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References
1.
Sluka KA, Kalra A, Moore SA: Unilateral intramuscular injections of acidic saline produce a bilateral, long-lasting hyperalgesia. Muscle Nerve 2001;24:37–46.
2.
Kang H, Woo YC, Baek CW, et al.: The effect of minocycline on allodynia produced by repeated injection of low pH saline in rats. Korean J Anesthesiol 2007;53:97–103.
3.
Nah SY: Ginseng: recent advances and Trends. J Ginseng Res 1997;21:1–12.
4.
Kaku T, Miyata T, Uruno T, Sako I, Kinoshita A: Chemico-pharmacological studies on saponins of Panax ginseng C. A. Meyer. II. Pharmacological part. Arzneimittelforschung 1975;25:539–547.
5.
Shin YH, Jung OM, Nah JJ, et al.: Ginsenosides that produce differential antinociception in mice. Gen Pharmacol 1999;32:653–659.
6.
Nah JJ, Hahn JH, Chung S, et al.: Effect of ginsenosides, active components of ginseng, on capsaicin-induced pain-related behavior. Neuropharmacology 2000;39:2180–2184.
7.
Gopalkrishnan P, Sluka KA: Effect of varying frequency, intensity and pulse duration of TENS on primary hyperalgesia in inflamed rats. Arch Phys Med Rehabil 2000;81:984–990.
8.
Shin YH, Kim SC, Han JW, et al.: Study on ginseng rotopanaxadiol and protopanaxatriol saponins-induced antinociception. Korean J Physiol Pharmacol 1997;1:143–149.
9.
Mogil JS, Shin YH, McCleskey EW, Kim SC, Nah SY: Ginsenoside Rf, a trace component of ginseng root, produces antinociception in mice. Brain Res 1998;792:218–228.
10.
Shin DJ, Yoon MH, Lee HG, et al.: The Effect of treatment with intrathecal ginsenosides in a rat model of postoperative pain. Korean J Pain 2007;20:100–105.
11.
Nah SY, McCleskey EW: Ginseng root extract inhibits calcium channels in rat sensory neurons through a similar path, but different receptor, as μ-type opioids. J Ethnopharmacol 1994;42:45–51.
12.
Go VL, Yaksh TL: Release of substance P from the cat spinal cord. J Physiol 1987;391:141–167.
13.
Skyba DA, King EW, Sluka KA: Effects of NMDA and non-NMDA ionotropic glutamate receptor antagonists on the development and maintenance of hyperalgesia induced by repeated intramuscular injection of acidic saline. Pain 2002;98:69–78.
14.
Chizh BA, Headley PM: NMDA antagonists and neuropathic pain—multiple drug targets and multiple uses. Curr Pharm Des 2005;11:2977–2994.
15.
Haley JE, Sullivan AF, Dickenson AH: Evidence for spinal N-methyl-D-aspartate receptor involvement in prolonged chemical nociception in the rat. Brain Res 1990;518:218–226.
16.
Davies SN, Lodge D: Evidence for involvement of N-methyl-D-aspartate receptors in “windup” of class 2 neurones in the dorsal horn of the rat. Brain Res 1987;424:402–406.
17.
Kim HS, Hwang SL, Oh S: Ginsenoside Rc and Rg1 differentially modulate NMDA receptor subunit mRNA levels after intracerebroventricular infusion in rats. Neurochem Res 2000;25:1149–1154.
18.
Nah JJ, Choi S, Kim YH, et al.: Effect of spinally administered ginseng total saponin on capsaicin-induced pain and excitatory amino acid-induced nociceptive responses. J Ginseng Res 1999;23:38–43.
19.
Yoon SR, Nah JJ, Shin YH, et al.: Ginsenosides induce differential antinociception and inhibit substance P induced-nociceptive response in mice. Life Sci 1998;62:319–325.
20.
Bhargava HN, Ramarao P: The effect of Panax ginseng on the development of tolerance to the pharmacological actions of morphine in the rat. Gen Pharmacol 1991;22:429–434.
21.
Watanabe J, Oh KW, Kim HS, Takahashi MT, Kaneto H: A non-opioid mechanism in the inhibitory effect of ginseng saponins on electrically evoked contractions of guinea pig ileum and mouse vas deferens. J Pharmacobiodyn 1988;11:453–458.
22.
Coon JT, Ernst E: Panax ginseng: a systematic review of adverse effects and drug interactions. Drug Saf 2002;25:323–344.
23.
Shin TJ, Hwang SH, Choi SH, et al.: Effects of protopanaxatriol-ginsenoside metabolites on rat N-methyl-D-aspartic Acid receptor-mediated ion currents. Korean J Physiol Pharmacol 2012;16:113–118.
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Copyright 2014, Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition.
History
Published online: 12 June 2014
Published in print: June 2014
Published ahead of print: 22 May 2014
Accepted: 8 March 2014
Received: 28 May 2013
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