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Published Online: 14 March 2023

Humulus japonicus Extract Ameliorates Hepatic Steatosis Through the PPARα-Mediated Suppression of Alcohol-Induced Oxidative Stress

Publication: Journal of Medicinal Food
Volume 26, Issue Number 3

Abstract

Humulus japonicus has been used to treat obesity, hypertension, and nonalcoholic fatty liver and to alleviate inflammation and oxidative stress. In the present study, we aimed to investigate the effects of H. japonicus ethanol extracts (HE) and luteolin 7-O-β-d-glucoside (LU), which is identified as a major active component of H. japonicus, on ethanol-induced oxidative stress and lipid accumulation in primary hepatocytes. Mouse primary hepatocytes were treated with HE and stimulated with ethanol. The MTT test was used to determine cell viability. By using Western blotting, the effects of HE on the expression of different proteins were investigated. Experimental mice were given a 5% alcohol liquid Lieber–DeCarli diet to induce alcoholic fatty liver. We found that both HE and LU individually attenuated ethanol-induced lipid accumulation, lipogenic protein expression, and cellular oxidative stress in hepatocytes. Treatment with HE or LU increased PPARα and SOD1 expression and catalase activity in a dose-dependent manner. Small interfering RNA of PPARα reduced the effects of HE on oxidative stress, lipid metabolism, and levels of antioxidants. We also observed that orally administered HE treatment alleviated hepatic steatosis in a diet containing ethanol-fed mice. This study suggests HE as a functional food that can improve hepatic steatosis, thereby preventing hepatic injury caused by alcohol consumption.

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cover image Journal of Medicinal Food
Journal of Medicinal Food
Volume 26Issue Number 3March 2023
Pages: 193 - 200
PubMed: 36827085

History

Published online: 14 March 2023
Published in print: March 2023
Published ahead of print: 23 February 2023
Accepted: 2 January 2023
Received: 2 August 2022

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Wonjun Cho*
Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul, Korea.
Seung Yeon Park*
Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul, Korea.
Department of Global Innovative Drugs, Graduate School of Chung-Ang University, Seoul, Korea.
Heeseung Oh
Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul, Korea.
A.M. Abd El-Aty
Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Department of Medical Pharmacology, Medical Faculty, Ataturk University, Erzurum, Turkiye.
Ahmet Hacimüftüoğlu
Department of Medical Pharmacology, Medical Faculty, Ataturk University, Erzurum, Turkiye.
Vaccine Development Application and Research Center, Ataturk University, Erzurum, Turkiye.
Dae-Sung Kim
Hanpoong Pharm and Foods Co., Ltd., Wanju, Korea.
Tae Woo Jung [email protected]
Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul, Korea.
Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul, Korea.
Department of Global Innovative Drugs, Graduate School of Chung-Ang University, Seoul, Korea.

Notes

*
These authors contributed equally to this work.
Address correspondence to: Ji Hoon Jeong, PhD, Department of Pharmacology, College of Medicine, Chung-Ang University, 221, Heuksuk-dong, Dongjak-gu, Seoul 156-756, Republic of Korea. [email protected]
Address correspondence to: Tae Woo Jung, PhD, Department of Pharmacology, College of Medicine, Chung-Ang University, 221, Heuksuk-dong, Dongjak-gu, Seoul 156-756, Republic of Korea. [email protected]

Authors' Contributions

W.C.: Conceptualization; investigation; and methodology. S.Y.P.: Conceptualization; investigation; and methodology. H.O. and D.-S.K.: Conceptualization; investigation; and methodology. J.H.J.: Conceptualization and roles/writing—original draft. T.W.J.: Conceptualization; data curation; formal analysis; funding acquisition; investigation; and roles/writing—original draft. A.H. and A.M.A.E.-A.: Roles/writing—original draft and writing—review and editing. All authors approved the final version of the article. All authors are responsible for the overall integrity of the work.

Author Disclosure Statement

No competing financial interests exist.

Funding Information

This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. 2021R1F1A1050004), (No. 2022R1A2B5B01001453) and by the Chung-Ang University Research Grants in 2022.

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