Research Article
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Published Online: 17 October 2007

Inhibition of Endotoxin-Induced Uveitis by Methylprednisolone Acetate Nanosuspension in Rabbits

Publication: Journal of Ocular Pharmacology and Therapeutics
Volume 23, Issue Number 5

Abstract

Purpose: In this study, nanoformulations of methylprednisolone acetate (MPA) were formulated by using a copolymer of poly(ethylacrylate, methyl–methacrylate and chlorotrimethyl–ammonioethyl methacrylate) to study their impacts on the inhibition of inflammatory symptoms in rabbits with endotoxin-induced uveitis (EIU).
Methods: A modified quasiemulsion solvent diffusion technique was used for the preparation of the nanoparticles. The drug-release profiles and physicochemical characteristics of the nanoformulations were studied by means of X-ray crystallography, differential scanning calorimetry, and Fourier transform infrared spectroscopy. Particle-size analysis yielded mean diameters of approximately 380, 460, and 580 (nm) for copolymer nanoparticles at the ratios of 1:2.5, 1:5, and 1:10, respectively. Major clinical symptoms of EIU (e.g., morphologic changes, leukocytes numbers, and protein levels within the aqueous humor) were examined.
Results: Upon the physicochemical characterizations, no crystal changes or chemical interactions were observed for the copolymer nanoparticles. The 1:2.5 ratio of drug polymer resulted in the most controlled release of MPA. The in vivo examinations revealed that the endotoxin-induced inflammation can be inhibited by the copolymer nanosuspension more significantly than by the microsuspension of MPA itself in the rabbits with EIU.
Conclusions: Based on our findings, we suggest that the copolymer nanosuspension may favor the localized, controlled ocular delivery of MPA for the prevention of inflammatory symptoms in ocular diseases.

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cover image Journal of Ocular Pharmacology and Therapeutics
Journal of Ocular Pharmacology and Therapeutics
Volume 23Issue Number 5October 2007
Pages: 421 - 432
PubMed: 17900230

History

Published online: 17 October 2007
Published in print: October 2007
Published ahead of print: 27 September 2007

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Khosro Adibkia
Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
Yadollah Omidi
Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Mohammad R. Siahi
Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Ali R. Javadzadeh
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Mohammad Barzegar-Jalali
Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Jaleh Barar
Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Nasrin Maleki
Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Ghobad Mohammadi
Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Ali Nokhodchi
Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Medway School of Pharmacy, University of Greenwich, Kent, United Kingdom.

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