Development of Direct LDL Measurement by LDL Precipitation Combined with Sandwich ELISA Using In-House-Generated mAbs
Publication: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
Volume 42, Issue Number 4
Abstract
One of the major risk factors for the progression of cardiovascular diseases is a high blood level of low-density lipoprotein (LDL), so progression of the disease may be prevented by lowering the plasma LDL. Evaluation and monitoring of LDL concentrations are therefore necessary. Friedewald's equation is a calculation method that is currently used routinely to estimate plasma LDL concentrations in hospital laboratories. However, this method cannot be applied to samples with a high concentration of triglyceride (TG). To overcome this limitation, this study aimed to develop direct LDL measurements using in-house generated monoclonal antibodies against human LDL in combination with LDL precipitation using heparin-containing citrate buffer pH 5.04. The method was applied to measure the LDL concentration in 208 randomized samples from Suranaree University of Technology Hospital. The mean values obtained from the developed method and the hospital laboratory were 126.6 ± 43.1 mg/dL and 123.2 ± 42.3 mg/dL, respectively. Linear regression analysis showed a high correlation between these two methods (r = 0.8491, p < 0.0001). High concentrations of TG, total cholesterol, and HDL have no influence on the LDL values obtained by this method. In this study, we offer an alternative technique for the direct measurement of plasma LDL. Further development for more convenient and easy use can now be undertaken.
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Information & Authors
Information
Published In
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
Volume 42 • Issue Number 4 • August 2023
Pages: 132 - 139
PubMed: 37581494
Copyright
Copyright 2023, Mary Ann Liebert, Inc., publishers.
History
Published online: 25 August 2023
Published ahead of print: 11 August 2023
Published in print: August 2023
Accepted: 23 July 2023
Received: 2 June 2023
Authors
Authors' Contributions
K.L.: conceptualization (equal); investigation (equal); methodology (equal); and writing—original draft (equal). P.K.: supervision (lead); conceptualization (equal); investigation (equal); methodology (equal); writing—original draft (equal); writing—review and editing (lead); and funding acquisition (lead).
Author Disclosure Statement
No competing financial interests exist.
Funding Information
This work was financially supported by SUT, Thailand Research and Innovation (TSRI), National Research Council of Thailand (no. SUT1-102-56-36-24), National Science Research and Innovation Fund (NSRF no. 160335 and no. 90464) and Biochemistry and Electrochemistry Research Unit.
Ethical Approval
This work was conducted according to the guidelines for handling human samples based on the Declaration of Helsinki. The documentation required for human studies was approved by the Ethics Committee for Researchers Involving Human Subjects, SUT, Nakhon Ratchasima, Thailand on March 4, 2013 and May 3, 2022.
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