Mitochondrial and Metabolic Dynamics of Endometrial Stromal Cells During the Endometrial Cycle
Publication: Stem Cells and Development
Volume 29, Issue Number 21
Abstract
The endometrial cycle in response to hormonal stimulation is essential for implantation. The female has endometrium that repeats this cycle through about half of a lifetime. The cycle includes three phases, proliferative, secretory, and menstrual, and each phase has distinct characteristics. The endometrial stromal cells (EnSCs) in each phase also have specialized characteristics, including cell cycle, morphologies, and cellular metabolic state. So we hypothesized that the cells in each phase have unique mitochondrial morphologies because they are generally linked to cellular metabolic state. To investigate the metabolic characteristics in each phase, we investigated the mitochondrial morphologies by transmission electron microscopy, oxygen consumption rate (OCR), and intracellular adenosine triphosphate (ATP) production. The decidualized EnSCs have shorter mitochondria than those in the proliferative phase. Besides, they also displayed distinct intracellular structural characteristics compared with the proliferative phase, such as ribosome-rich endoplasmic reticulum and increased formation of vesicles. OCR and luminescent ATP detection assay revealed that the basal respiration and ATP production in the decidualized EnSCs were lower than those in the proliferative phase. Thus, we concluded that morphological and intracellular structural changes were induced during the decidualization. Moreover, the decreased mitochondrial length was shown to correlate with decreased dependency on oxidative phosphorylation and ATP concentration in EnSCs.
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Information & Authors
Information
Published In
Stem Cells and Development
Volume 29 • Issue Number 21 • November 1, 2020
Pages: 1407 - 1415
PubMed: 32867608
Copyright
Copyright 2020, Mary Ann Liebert, Inc., publishers.
History
Published in print: November 1, 2020
Published online: 28 October 2020
Published ahead of print: 24 September 2020
Published ahead of production: 31 August 2020
Accepted: 31 August 2020
Received: 29 July 2020
Authors
Author Disclosure Statement
No competing financial interests exist.
Funding Information
This study was supported by the National Research Foundation of Korea (NRF) grant funded by the government (MSIT) of the Republic of Korea (grant nos. 2016M3A9B6946835, 2015R1A5A1009701, and 2020R1A2C3007562).
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