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Published Online: 11 November 2021

Lrrc34 Interacts with Oct4 and Has an Impact on Telomere Length in Mouse Embryonic Stem Cells

Publication: Stem Cells and Development
Volume 30, Issue Number 22

Abstract

Telomere length maintenance in pluripotent stem cells (PSCs) is a main characteristic and a major premise for their undifferentiated long-term survival. However, little is known about the factors that control telomere length and elongation in these cells. Here, I describe Lrrc34 (leucine-rich repeat 34) as a novel telomere length regulating gene in murine embryonic stem cells. Downregulation of Lrrc34 results in significant reduction of telomerase activity and telomere length over time while also influencing the expression of known telomere length-associated genes. Generating induced PSCs (iPSCs) with Lrrc34 as a fifth factor in classical Yamanaka reprogramming increases the efficiency but did not have an impact on telomere length in the resulting iPSCs. Moreover, Lrrc34 was found to interact with Oct4, connecting the pluripotency network to telomere length regulation.

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Information & Authors

Information

Published In

cover image Stem Cells and Development
Stem Cells and Development
Volume 30Issue Number 22November 2021
Pages: 1093 - 1102
PubMed: 34549596

History

Published in print: November 2021
Published online: 11 November 2021
Published ahead of print: 27 October 2021
Published ahead of production: 22 September 2021
Accepted: 16 September 2021
Received: 25 May 2021

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    Jessica Nolte [email protected]
    Institute of Human Genetics, University Medical Center Göttingen, Göttingen, Germany.

    Notes

    Address correspondence to: Dr. Jessica Nolte, Institute of Human Genetics, University Medical Center Göttingen, Heinrich-Düker-Weg 12, Göttingen 37073, Germany [email protected]

    Author Disclosure Statement

    No competing financial interests exist.

    Funding Information

    This work was supported by the German Research Foundation (DFG) grant to Jessica Nolte (No. 941/4-1).

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