Research Article
No access
Published Online: 16 January 2007

Design of Biomimetic Habitats for Tissue Engineering with P-15, a Synthetic Peptide Analogue of Collagen

Publication: Tissue Engineering
Volume 5, Issue Number 1

Abstract

In tissues, collagen forms the scaffold for cell attachment and migration, and it modulates cell differentiation and morphogenesis by mediating the flux of chemical and mechanical stimuli. We are constructing biomimetic environments by immobilizing a collagen-derived high-affinity cell-binding peptide P-15 in three-dimensional (3-D) templates. The cell-binding peptide can be expected to transduce mechanical forces. In their physiological environment, periodontal ligament fibroblasts (PDLF) are subject to significant mechanical forces. We have examined the behavior of human PDLF in culture on particulate bovine anorganic bone mineral (ABM) coated with P-15 (ABM–P-15). Greater numbers of cells associated with ABM–P-15 compared to ABM alone. Higher levels of incorporation of radiolabeled precursors in DNA and protein were consistent with the presence of larger numbers of cells on ABM–P-15 compared to ABM cultures. Scanning electron microscopic examination showed that cultures on ABM–P-15 generated highly oriented 3-D colonies of elongated cells and formed copious amounts of fibrous as well as membranous matrix reminiscent of ligamentous structures. PDLF cultured on ABM formed sparse monolayers with little order and a meager matrix. Alizarin Red stained the matrix of particle associated cells and inter-particle cellular bridges in P-15-associated cultures, indicating mineralization. 3-D colony formation and ordering of cells along with increased mineralization suggests that the coupling of cells to the ABM matrix through P-15 may provide a biomimetic environment permissive for cell differentiation and morphogenesis. Our studies suggest that ABM–P-15 templates may be effective as endosseous grafts, and, when seeded with PDLF, these matrices may serve as tissue engineered substitutes for autologous bone grafts.

Get full access to this article

View all available purchase options and get full access to this article.

Information & Authors

Information

Published In

cover image Tissue Engineering
Tissue Engineering
Volume 5Issue Number 1February 1999
Pages: 53 - 65
PubMed: 10207189

History

Published online: 16 January 2007
Published in print: February 1999

Permissions

Request permissions for this article.

Topics

Authors

Affiliations

Rajendra S. Bhatnagar
Laboratory of Connective Tissue Biochemistry, School of Dentistry, University of California, San Francisco, and Department of Bioengineering, University of California San Francisco and Berkeley, California.
Jing Jing Qian
Laboratory of Connective Tissue Biochemistry, School of Dentistry, University of California, San Francisco, and Department of Bioengineering, University of California San Francisco and Berkeley, California.
Anna Wedrychowska
Laboratory of Connective Tissue Biochemistry, School of Dentistry, University of California, San Francisco, and Department of Bioengineering, University of California San Francisco and Berkeley, California.
Mahyar Sadeghi
Periodontics and Oral Biology, School of Dentistry, University of California, San Francisco, California.
Yi Min Wu
Laboratory of Connective Tissue Biochemistry, School of Dentistry, University of California, San Francisco, and Department of Bioengineering, University of California San Francisco and Berkeley, California.
Nancy Smith
Microscope and Graphical Imaging Center, California State University, Hayward, California.

Metrics & Citations

Metrics

Citations

Export citation

Select the format you want to export the citations of this publication.

View Options

Get Access

Access content

To read the fulltext, please use one of the options below to sign in or purchase access.

Society Access

If you are a member of a society that has access to this content please log in via your society website and then return to this publication.

Restore your content access

Enter your email address to restore your content access:

Note: This functionality works only for purchases done as a guest. If you already have an account, log in to access the content to which you are entitled.

View options

PDF/EPUB

View PDF/ePub

Media

Figures

Other

Tables

Share

Share

Copy the content Link

Share on social media

Back to Top