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Published Online: 13 January 2006

Synthesis and in Vitro Evaluation of Enzymatically Cross-Linked Elastin-Like Polypeptide Gels for Cartilaginous Tissue Repair

Publication: Tissue Engineering
Volume 11, Issue Number 11-12

Abstract

Genetically engineered elastin-like polypeptide (ELP) hydrogels offer unique promise as scaffolds for cartilage tissue engineering because of the potential to promote chondrogenesis and to control mechanical properties. In this study, we designed and synthesized ELPs capable of undergoing enzyme- initiated gelation via tissue transglutaminase, with the ultimate goal of creating an injectable, in situ cross-linking scaffold to promote functional cartilage repair. Addition of the enzyme promoted ELP gel formation and chondrocyte encapsulation in a biocompatible process, which resulted in cartilage matrix synthesis in vitro and the potential to contribute to cartilage mechanical function in vivo. A significant increase in the accumulation of sulfated glycosaminoglycans was observed, and histological sections revealed the accumulation of a cartilaginous matrix rich in type II collagen and lacking in type I collagen, indicative of hyaline cartilage formation. These results provide evidence of chondrocytic phenotype maintenance for cells in the ELP hydrogels in vitro. In addition, the dynamic shear moduli of ELP hydrogels seeded with chondrocytes increased from 0.28 to 1.7 kPa during a 4-week culture period. This increase in the mechanical integrity of cross-linked ELP hydrogels suggests restructuring of the ELP matrix by deposition of functional cartilage extracellular matrix components.

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Published In

cover image Tissue Engineering
Tissue Engineering
Volume 11Issue Number 11-12November/December 2005
Pages: 1768 - 1779
PubMed: 16411822

History

Published online: 13 January 2006
Published in print: November/December 2005

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Melissa K. McHale
Department of Biomedical Engineering, Duke University, Durham, North Carolina.
Lori A. Setton
Department of Biomedical Engineering, Duke University, Durham, North Carolina.
Ashutosh Chilkoti
Department of Biomedical Engineering, Duke University, Durham, North Carolina.

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