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Published Online: 21 December 2010

Platelet-Rich Fibrin is a Bioscaffold and Reservoir of Growth Factors for Tissue Regeneration

Publication: Tissue Engineering Part A
Volume 17, Issue Number 3-4

Abstract

The platelet-rich fibrin (PRF) is known as a rich source of autologous cytokines and growth factors and universally used for tissue regeneration in current clinical medicine. However, the microstructure of PRF has not been fully investigated nor have been studied the key molecules that differ PRF from platelet-rich plasma. We fabricated PRF under Choukroun's protocol and produced its extract (PRFe) by freezing at −80°C. The conventional histological, immunohistological staining, and scanning electron microscopy images showed the microstructure of PRF, appearing as two zones, the zone of platelets and the zone of fibrin, which resembled a mesh containing blood cells. The PRFe increased proliferation, migration, and promoted differentiation of the human alveolar bone marrow stem cells (hABMSCs) at 0.5% concentration in vitro. From the results of proteome array, matrix metalloproteinase 9 (MMP9) and Serpin E1 were detected especially in PRFe but not in concentrated platelet-rich plasma. Simultaneous elevation of MMP9, CD44, and transforming growth factor β-1 receptor was shown at 0.5% PRFe treatment to the hABMSC in immunoblot. Mineralization assay showed that MMP9 directly regulated mineralization differentiation of hABMSC. Transplantation of the fresh PRF into the mouse calvarias enhanced regeneration of the critical-sized defect. Our results strongly support the new characteristics of PRF as a bioscaffold and reservoir of growth factors for tissue regeneration.

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Published In

cover image Tissue Engineering Part A
Tissue Engineering Part A
Volume 17Issue Number 3-4February 2011
Pages: 349 - 359
PubMed: 20799908

History

Published in print: February 2011
Published ahead of print: 31 December 2010
Published online: 21 December 2010
Published ahead of production: 29 August 2010
Accepted: 27 August 2010
Received: 4 June 2010

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Young-Ho Kang
*
Department of Oral and Maxillofacial Surgery, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.
Soung Hoo Jeon*
Department of Oral and Maxillofacial Surgery, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.
Tooth Bioengineering National Research Laboratory, BK21, School of Dentistry, Seoul National University, Seoul, Korea.
Joo-Young Park
Department of Oral and Maxillofacial Surgery, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.
Tooth Bioengineering National Research Laboratory, BK21, School of Dentistry, Seoul National University, Seoul, Korea.
Jong-Hoon Chung
Department of Biosystems and Biomaterials Science and Engineering, College of Agriculture and Life Science, Seoul National University, Seoul, Korea.
Yun-Hoon Choung
Department of Otolaryngology, School of Medicine, Ajou University, Suwon, Korea.
Han-Wool Choung
Department of Oral and Maxillofacial Surgery, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.
Eun-Suk Kim
Department of Oral and Maxillofacial Surgery, School of Medicine, Choongnam National University, Tae-jun, Korea.
Pill-Hoon Choung
Department of Oral and Maxillofacial Surgery, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.
Tooth Bioengineering National Research Laboratory, BK21, School of Dentistry, Seoul National University, Seoul, Korea.

Notes

Address correspondence to:Pill-Hoon Choung, D.D.S., Ph.D.Department of Oral and Maxillofacial SurgeryDental Research InstituteSchool of DentistrySeoul National University28 Yeongeon-dong Jongno-guSeoul 110-749KoreaE-mail: [email protected]

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