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Published Online: 26 March 2013

Adipose-Derived Stem Cells Promote Angiogenesis and Tissue Formation for In Vivo Tissue Engineering

Publication: Tissue Engineering Part A
Volume 19, Issue Number 11-12

Abstract

Adult mesenchymal stem cells secrete a variety of angiogenic cytokines and growth factors, so we proposed that these paracrine mechanisms may be used to promote vascularization and growth for tissue engineering in vivo. We tested whether or not human adipose-derived stem cells (ASCs) promote tissue formation in rats. ASCs were evaluated in vitro for mRNA expression of angiogenic factors, including the vascular endothelial growth factor, basic fibroblast growth factor, interleukin-8 (IL-8), and stromal cell-derived factor-1 (SDF-1) and proliferative activity on human microvascular endothelial cells. For in vivo analysis, CM-DiI-labeled ASCs were implanted with a rat cardiac extracellular matrix (ECM) extract-derived hydrogel into a chamber with a femoral arteriovenous loop in the groin of male nude rats for 7 days. Vascularization in newly generated tissue was estimated by histomorphometry after endothelial nitric oxide synthase (eNOS) immunostaining. ASCs expressed growth factor mRNA and produced an angiogenic activity in vitro. After implantation, ASCs survived, but remained suspended in the ECM and relatively few were incorporated into the newly formed tissue. The volume of newly generated tissue was significantly higher in chambers containing ASCs and it was enriched with vasculature when compared with the ECM alone. We conclude that human ASCs promote tissue growth and angiogenesis in the rat vascularized chamber, thereby showing promise for tissue-engineering applications for regenerative therapy.

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cover image Tissue Engineering Part A
Tissue Engineering Part A
Volume 19Issue Number 11-12June 2013
Pages: 1327 - 1335
PubMed: 23394225

History

Published in print: June 2013
Published ahead of print: 28 March 2013
Published online: 26 March 2013
Published ahead of production: 8 February 2013
Accepted: 3 January 2013
Received: 26 June 2012

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Ken Matsuda, MD
*
O'Brien Institute, Fitzroy, Victoria, Australia.
Department of Surgery, St. Vincent's Hospital, University of Melbourne, Fitzroy, Victoria, Australia.
Katrina J. Falkenberg, BSc (Hons)*
O'Brien Institute, Fitzroy, Victoria, Australia.
Alan A. Woods, PhD
O'Brien Institute, Fitzroy, Victoria, Australia.
Yu Suk Choi, PhD
O'Brien Institute, Fitzroy, Victoria, Australia.
Department of Surgery, St. Vincent's Hospital, University of Melbourne, Fitzroy, Victoria, Australia.
Wayne A. Morrison, MD, FRACS
O'Brien Institute, Fitzroy, Victoria, Australia.
Department of Surgery, St. Vincent's Hospital, University of Melbourne, Fitzroy, Victoria, Australia.
Australian Catholic University, Fitzroy, Victoria, Australia.
Rodney J. Dilley, PhD
O'Brien Institute, Fitzroy, Victoria, Australia.
Department of Surgery, St. Vincent's Hospital, University of Melbourne, Fitzroy, Victoria, Australia.
Ear Science Institute Australia, School of Surgery, University of Western Australia, Nedlands, Western Australia, Australia.

Notes

Address correspondence to:Rodney J. Dilley, PhDEar Science Institute AustraliaEar Sciences CentreSchool of Surgery M509University of Western AustraliaNedlands, WA 6009Australia
E-mail: [email protected]

Disclosure Statement

Professor Morrison is an inventor on the Vascularized Tissue Graft patent and entitled to proceeds derived from commercialization of the patent, and is a board member and employee of the O'Brien Institute, which has an interest in the company charged with the commercialization of the Vascularized Tissue Graft patent. The remaining authors report no competing financial interests.

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