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Published Online: 29 January 2010

Facilitated Noninvasive Visualization of Collagen and Elastin in Blood Vessels

Publication: Tissue Engineering Part C: Methods
Volume 16, Issue Number 4

Abstract

Multiphoton imaging is a powerful tool for three-dimensional visualization of extracellular matrix components such as collagen and elastin in fresh, nonfixed, and nonembedded tissues. We have previously published data on the induction of the second harmonic generation signal of collagen and autofluorescence of elastin using a tunable multiphoton laser system. Without staining, a second harmonic generation signal was detected for collagen when excited at wavelength λex = 840 nm. Switching the excitation wavelength to 760 nm enabled visualization of elastic fiber structures. A limitation of this technology is the laser-tuning process that requires calibration of the system in between the studies. Now we have developed a facilitated method for studying tissues and tissue equivalents that enables simultaneous visualization of collagen and elastin structures using only a single excitation wavelength of 840 nm in combination with two different band-pass filters. This facilitated method will expand the range of application by reducing required time and expenses for the laser system without reducing its capability.

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Published In

cover image Tissue Engineering Part C: Methods
Tissue Engineering Part C: Methods
Volume 16Issue Number 4August 2010
Pages: 705 - 710
PubMed: 19803792

History

Published in print: August 2010
Published ahead of print: 13 April 2010
Published online: 29 January 2010
Published ahead of production: 5 October 2009
Accepted: 5 October 2009
Received: 5 May 2009

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    Affiliations

    Olaf Fritze
    Department of Thoracic, Cardiac, and Vascular Surgery, University Hospital Tuebingen, Tuebingen, Germany.
    Martina Schleicher
    Department of Thoracic, Cardiac, and Vascular Surgery, University Hospital Tuebingen, Tuebingen, Germany.
    Karsten König
    Faculty of Mechanotronics and Physics, Saarland University, Saarbruecken, Germany.
    Katja Schenke-Layland
    Cardiovascular Research Laboratory, David Geffen School of Medicine at UCLA, Los Angeles, California.
    Ulrich Stock
    Department of Thoracic, Cardiac, and Vascular Surgery, University Hospital Tuebingen, Tuebingen, Germany.
    Csaba Harasztosi
    Department of Otolaryngology, Head, and Neck Surgery, University Hospital Tuebingen, Tuebingen, Germany.

    Notes

    Address correspondence to:Olaf Fritze, Ph.D.Department of Thoracic, Cardiac and Vascular SurgeryUniversity Hospital TuebingenSilcher Strasse 7/1Tuebingen 72076Germany
    E-mail: [email protected]

    Disclosure Statement

    No competing financial interests exist.

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