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Published Online: 12 October 2010

PAM2 (Piston Assisted Microsyringe): A New Rapid Prototyping Technique for Biofabrication of Cell Incorporated Scaffolds

Publication: Tissue Engineering Part C: Methods
Volume 17, Issue Number 2

Abstract

Rapid prototyping techniques are widely used to fabricate well-defined three-dimensional structures of tissue homologs. The piston-assisted microsyringe (PAM2) is a rapid prototyping technology specifically developed for low-shear stress extrusion of viscous hydrogel solutions containing cells. In this article the working parameters of the system were established to guarantee the realization of spatially controlled hydrogel scaffolds. Moreover the shear stresses acting on the cell membrane during extrusion was investigated through a computational fluid-dynamic analysis. The computational models show that the shear stress on the cells is of the order of 100 Pa during the extrusion process. HepG2 cells encapsulated in alginate were then extruded into spatially organized hepatic lobule-like architectures and their viability and function were evaluated. The results show that the metabolic fingerprint of the cells is preserved with respect to controls and the cells are uniformly distributed through the gel scaffold.

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Information

Published In

cover image Tissue Engineering Part C: Methods
Tissue Engineering Part C: Methods
Volume 17Issue Number 2February 2011
Pages: 229 - 237
PubMed: 20799910

History

Published in print: February 2011
Published ahead of print: 19 October 2010
Published online: 12 October 2010
Published ahead of production: 29 August 2010
Accepted: 27 August 2010
Received: 29 March 2010

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Annalisa Tirella
Interdepartmental Research Center “E. Piaggio,” Faculty of Engineering, University of Pisa, Pisa, Italy.
Department of Chemical Science and Technology, University of Rome “Tor Vergata,” Roma, Italy.
Federico Vozzi
Laboratory of Biomimetic Materials and Tissue Engineering, Institute of Clinical Physiology-CNR, Pisa, Italy.
Giovanni Vozzi
Interdepartmental Research Center “E. Piaggio,” Faculty of Engineering, University of Pisa, Pisa, Italy.
Arti Ahluwalia
Interdepartmental Research Center “E. Piaggio,” Faculty of Engineering, University of Pisa, Pisa, Italy.

Notes

Address correspondence to:Arti Ahluwalia, Ph.D.Interdepartmental Research Center “E. Piaggio”Faculty of EngineeringUniversity of PisaVia Diotisalvi 2Pisa 56126Italy
E-mail: [email protected]

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No competing financial interests exist.

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