Research Article
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Published Online: 6 August 2014

An Electrohydrodynamic Bioprinter for Alginate Hydrogels Containing Living Cells

Publication: Tissue Engineering Part C: Methods
Volume 21, Issue Number 2

Abstract

In this work we present a bioprinting technique that exploits the electrohydrodynamic process to obtain a jet of liquid alginate beads containing cells. A printer is used to microfabricate hydrogels block by block following a bottom-up approach. Alginate beads constitute the building blocks of the microfabricated structures. The beads are placed at predefined position on a target substrate made of calcium-enriched gelatin, where they crosslink upon contact without the need of further postprocessing. The printed sample can be easily removed from the substrate at physiological temperature. Three-dimensional printing is accomplished by the deposition of multiple layers of hydrogel. We have investigated the parameters influencing the process, the compatibility of the printing procedure with cells, and their survival after printing.

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Information & Authors

Information

Published In

cover image Tissue Engineering Part C: Methods
Tissue Engineering Part C: Methods
Volume 21Issue Number 2February 2015
Pages: 123 - 132
PubMed: 24903714

History

Published in print: February 2015
Published ahead of print: 8 August 2014
Published online: 6 August 2014
Published ahead of production: 6 June 2014
Accepted: 30 May 2014
Received: 7 March 2014

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Luca Gasperini
Department of Industrial Engineering, Biotech Research Center, University of Trento, Trento, Italy.
European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Trento, Italy.
Devid Maniglio
Department of Industrial Engineering, Biotech Research Center, University of Trento, Trento, Italy.
European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Trento, Italy.
INSTM—Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali, Firenze, Italy.
Antonella Motta
Department of Industrial Engineering, Biotech Research Center, University of Trento, Trento, Italy.
European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Trento, Italy.
INSTM—Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali, Firenze, Italy.
Claudio Migliaresi
Department of Industrial Engineering, Biotech Research Center, University of Trento, Trento, Italy.
European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Trento, Italy.
INSTM—Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali, Firenze, Italy.

Notes

Address correspondence to:Claudio Migliaresi, PhDDepartment of Industrial EngineeringUniversity of TrentoVia Sommarive 938123 TrentoItaly
E-mail: [email protected]

Disclosure Statement

No competing financial interests exist.

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