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Published Online: 1 September 2017

Supercritical Carbon Dioxide-Assisted Decellularization of Aorta and Cornea

Publication: Tissue Engineering Part C: Methods
Volume 23, Issue Number 9


Tissue engineering approaches utilize both natural and synthetic materials in the repair and regeneration processes. A naturally sourced material for this purpose is required to be free from any antigenic matter such as cells or cellular components. Decellularization of tissues may be achieved through chemical or physical removal agents. Supercritical carbon dioxide (sc-CO2) has been used on the purpose of removing bioburden from tissues and offers an alternative to the traditionally used treatment methods. In addition to many advantages it offers with regard to the successful decellularization of tissues, it is known to have a sterilization effect. This study provides an insight into sc-CO2–assisted decellularization trials of corneal and aortic tissues. Results showed that high pressure of the fluid bursts the cells during the treatment and rapid depressurization was found to be effective in the removal of the cells from the tissues. sc-CO2 decellularization offers significantly reduced treatment times, complete decellularization, and preserved extracellular matrix structure.

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Published In

cover image Tissue Engineering Part C: Methods
Tissue Engineering Part C: Methods
Volume 23Issue Number 9September 2017
Pages: 540 - 547
PubMed: 28726559


Published in print: September 2017
Published online: 1 September 2017
Published ahead of print: 17 August 2017
Published ahead of production: 20 July 2017
Accepted: 5 July 2017
Received: 22 February 2017


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Selcan Guler
Bioengineering Division, Hacettepe University, Ankara, Turkey.
Bahar Aslan
Bioengineering Division, Hacettepe University, Ankara, Turkey.
Pezhman Hosseinian
Nanotechnology and Nanomedicine Division, Institute of Science, Hacettepe University, Ankara, Turkey.
Halil Murat Aydin
Bioengineering Division, Environmental Engineering Department, Centre for Bioengineering, Hacettepe University, Ankara, Turkey.


Address correspondence to:Halil Murat Aydin, PhDBioengineering DivisionEnvironmental Engineering DepartmentCentre for BioengineeringHacettepe UniversityAnkara 06800Turkey
E-mail: [email protected]

Disclosure Statement

No competing financial interests exist.

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