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Published Online: 6 July 2010

WDR3 Gene Haplotype Is Associated with Thyroid Cancer Risk in a Spanish Population

Publication: Thyroid
Volume 20, Issue Number 7

Abstract

Background: A member of the genes encoding WD-repeat proteins, the WDR3 gene, maps in the 1p12 region. This region was shown to be associated with thyroid cancer susceptibility in a previous work. In this study we aim to evaluate the contribution of WDR3 to thyroid cancer risk.
Methods: A case–control association study was performed in a total of 402 patients and 479 control subjects from a Spanish population. In the initial phase of the study, 10 single-nucleotide polymorphisms covering the WDR3 region were genotyped in a small group (157 patients and 118 control subjects); next, three of the initial single-nucleotide polymorphisms were further genotyped in the overall population. In addition, WDR3 expression was investigated in 10 thyroid cancer cell lines by RT-PCR and Western blot.
Results: Haplotype analysis revealed that combination of certain WDR3 variants, such as haplotype CAT, increases the risk of thyroid cancer (odds ratio = 1.85, 95% confidence interval = 0.97–3.55, p = 0.063). Further, both messenger RNA transcription and protein expression of WDR3 were altered in human thyroid cancer cells.
Conclusion: These results indicate for the first time that WDR3 is a risk factor to thyroid cancer, suggesting its implication in the etiology of thyroid cancer.

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Published In

cover image Thyroid®
Thyroid
Volume 20Issue Number 7July 2010
Pages: 803 - 809
PubMed: 20578902

History

Published online: 6 July 2010
Published in print: July 2010
Published ahead of print: 26 June 2010

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Abdelmounaim Akdi
Group of Mutagenesis, Department of Genetics and Microbiology, Faculty of Bioscience, Autonomous University of Barcelona, Bellaterra, Spain.
Esteban-Mariano Giménez
Group of Mutagenesis, Department of Genetics and Microbiology, Faculty of Bioscience, Autonomous University of Barcelona, Bellaterra, Spain.
Wilser García-Quispes
Group of Mutagenesis, Department of Genetics and Microbiology, Faculty of Bioscience, Autonomous University of Barcelona, Bellaterra, Spain.
Susana Pastor
Group of Mutagenesis, Department of Genetics and Microbiology, Faculty of Bioscience, Autonomous University of Barcelona, Bellaterra, Spain.
CIBER Epidemiology and Public Health, ISCIII, Barcelona, Spain.
Juan Castell
Nuclear Medicine Services, Vall d′Hbron Hospital, Barcelona, Spain.
Josefina Biarnés
Endocrinology Unit, Josep Trueta Hospital, Girona, Spain.
Ricard Marcos
Group of Mutagenesis, Department of Genetics and Microbiology, Faculty of Bioscience, Autonomous University of Barcelona, Bellaterra, Spain.
CIBER Epidemiology and Public Health, ISCIII, Barcelona, Spain.
Antonia Velázquez
Group of Mutagenesis, Department of Genetics and Microbiology, Faculty of Bioscience, Autonomous University of Barcelona, Bellaterra, Spain.
CIBER Epidemiology and Public Health, ISCIII, Barcelona, Spain.

Notes

Address correspondence to:Antonia Velázquez, Ph.D.Departament de Genètica i de MicrobiologiaEdifici CnUniversitat Autònoma de Barcelona08193 BellaterraSpain
E-mail: [email protected]

Disclosure Statement

The authors declare that no competing financial interests exist.

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