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Published Online: 2 July 2013

Detection of Rickettsia parkeri from within Piura, Peru, and the First Reported Presence of Candidatus Rickettsia andeanae in the Tick Rhipicephalus sanguineus

Publication: Vector-Borne and Zoonotic Diseases
Volume 13, Issue Number 7

Abstract

Domestic farm animals (n=145) were sampled for the presence of ectoparasites in northwestern Peru during March, 2008. Ninety domestic animals (62%) were positive for the presence of an ectoparasite(s) and produced a total collection of the following: 728 ticks [Amblyomma maculatum, Anocentor nitens, Rhipicephalus (Boophilus) microplus, Rhipicephalus sanguineus, and Otobius megnini], 12 lice (Haematopinus suis), and 3 fleas (Ctenocephalides felis). A Rickettsia genus-specific qPCR assay was performed on nucleic acid preparations of the collected ectoparasites that resulted in 5% (37/743, 35 ticks and 2 fleas) of the ectoparasites positive for the presence of Rickettsia. DNA from the positive individual ticks was tested with 2 other qPCR assays for the presence of the ompB gene in Candidatus Rickettsia andeanae or Rickettsia parkeri. Candidatus R. andeanae was found in 25 A. maculatum ticks and in two Rh. sanguineus ticks, whereas R. parkeri was detected in 6 A. maculatum ticks. Two A. maculatum were co-infected with both Candidatus R. andeanae and R. parkeri. Rickettsia felis was detected in 2 fleas, Ctenocephalides felis, by multilocus sequence typing of the 17-kD antigen and ompA genes. These findings expand the geographic range of R. parkeri to include Peru as well as expand the natural arthropod vector of Candidatus R. andeanae to include Rhipicephalus sanguineus.

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cover image Vector-Borne and Zoonotic Diseases
Vector-Borne and Zoonotic Diseases
Volume 13Issue Number 7July 2013
Pages: 505 - 508
PubMed: 23488453

History

Published online: 2 July 2013
Published in print: July 2013
Published ahead of print: 14 March 2013

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Carmen Flores-Mendoza
US Naval Medical Research Unit No. Six, Lima, Peru, Department of Entomology, Washington, DC.
David Florin
US Naval Medical Research Unit No. Six, Lima, Peru, Department of Entomology, Washington, DC.
Department of Preventive Medicine and Biometrics, Uniformed Services University of the Health Services, Bethesda, Maryland.
Vidal Felices
US Naval Medical Research Unit No. Six, Lima, Peru, Department of Entomology, Washington, DC.
Edwar J. Pozo
Ministry of Health, Sullana, Peru.
Paul C.F. Graf
US Naval Medical Research Unit No. Six, Lima, Peru, Department of Entomology, Washington, DC.
University of Rochester Medical Center, Clinical Microbiology Laboratory, Rochester, New York.
Roxanne G. Burrus
US Naval Medical Research Unit No. Six, Lima, Peru, Department of Entomology, Washington, DC.
Allen L. Richards
Department of Preventive Medicine and Biometrics, Uniformed Services University of the Health Services, Bethesda, Maryland.
Naval Medical Research Center, Silver Spring, Maryland.

Notes

Address correspondence to:Roxanne G. BurrusU.S. Naval Medical Research Unit No. Six (NAMRU-6)Avenida Venezuela Cuadra 36Callao 2Peru
E-mail: [email protected]

Author Disclosure Statement

No competing financial interests exist.

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