Molecular Detection of Candidatus Bartonella mayotimonensis in North American Bats
Publication: Vector-Borne and Zoonotic Diseases
Volume 17, Issue Number 4
Abstract
Candidatus Bartonella mayotimonensis was detected in 2010 from an aortic valve sample of a patient with endocarditis from Iowa, the United States of America. The environmental source of the potentially new endocarditis-causing Bartonella remained elusive. We set out to study the prevalence and diversity of bat-associated Bartonella in North America. During 2015, mist nets and harp traps were used to capture 92 bats belonging to two species: little brown myotis (Myotis lucifugus Le Conte 1831, n = 73) and the gray myotis (M. grisescens A.H. Howell 1909, n = 19) in Kentucky, Michigan, Pennsylvania, and Tennessee. DNA preparations of peripheral blood samples from bats were subjected to a three-marker (gltA, rpoB, and intergenic spacer region [ISR]) multilocus sequence analysis. Sequence-verified gltA-positive PCR amplicons were obtained from nine samples. Three sequences were 99.7–100% identical with the gltA sequence of the Iowa endocarditis patient strain. Analysis of rpoB and ISR sequences demonstrated that one little brown myotis sample from the Upper Peninsula of Michigan contained Bartonella DNA, with 100% sequence identity with the Iowa endocarditis patient strain DNA. It appears possible that bats are a reservoir of Candidatus Bartonella mayotimonensis in North America.
Get full access to this article
View all available purchase options and get full access to this article.
References
Bai Y, Kosoy M, Recuenco S, Alvarez D, et al. Bartonella spp. in bats, Guatemala. Emerg Infect Dis 2011; 17:1269–1272.
Bai Y, Recuenco S, Gilbert AT, Osikowicz LM, et al. Prevalence and diversity of Bartonella spp. in bats in Peru. Am J Trop Med Hyg 2012; 87:518–523.
Concannon R, Wynn-Owen K, Simpson VR, Birtles RJ. Molecular characterization of haemoparasites infecting bats (Microchiroptera) in Cornwall, UK. Parasitology 2005; 131:489–496.
Edouard S, Nabet C, Lepidi H, Fournier PE, et al. Bartonella, a common cause of endocarditis: A report on 106 cases and review. J Clin Microbiol 2015; 53:824–829.
García-Esteban C, Gil H, Rodríguez-Vargas M, Gerrikagoitia X, et al. Molecular method for Bartonella species identification in clinical and environmental samples. J Clin Microbiol 2008; 46:776–779.
Gill JS, Rowley WA, Bush PJ, Viner JP, et al. Detection of human blood in the bat tick Carios (Ornithodoros) kelleyi (Acari: Argasidae) in Iowa. J Med Entomol 2004; 41:1179–1181.
Kosoy M, Bai Y, Lynch T, Kuzmin IV, et al. Bartonella spp. in bats, Kenya. Emerg Infect Dis 2010; 16:1875–1881.
Lilley TM, Veikkolainen V, Pulliainen AT. Molecular detection of Candidatus Bartonella hemsundetiensis in bats. Vector Borne Zoonotic Dis 2015; 15:706–708.
Lin EY, Tsigrelis C, Baddour LM, Lepidi H, et al. Candidatus Bartonella mayotimonensis and endocarditis. Emerg Infect Dis 2010; 16:500–503.
Lin JW, Hsu YM, Chomel BB, Lin LK, et al. Identification of novel Bartonella spp. in bats and evidence of Asian gray shrew as a new potential reservoir of Bartonella. Vet Microbiol 2012; 156:119–126.
Loftis AD, Gill JS, Schriefer ME, Levin ML, et al. Detection of Rickettsia, Borrelia, and Bartonella in Carios kelleyi (Acari: Argasidae). J Med Entomol 2005; 42:473–480.
Mannerings AO, Osikowicz LM, Restif O, Nyarko E, et al. Exposure to bat-associated Bartonella spp. among humans and other animals, Ghana. Emerg Infect Dis 2016; 22:922–924.
Norman AF, Regnery R, Jameson P, Greene C, et al. Differentiation of Bartonella-like isolates at the species level by PCR-restriction fragment length polymorphism in the citrate synthase gene. J Clin Microbiol 1995; 33:1797–1803.
Pulliainen AT, Dehio C. Persistence of Bartonella spp. stealth pathogens: From subclinical infections to vasoproliferative tumor formation. FEMS Microbiol Rev 2012; 36:563–599.
Reeves WK, Loftis AD, Gore JA, Dasch GA. Molecular evidence for novel Bartonella species in Trichobius major (Diptera: Streblidae) and Cimex adjunctus (Hemiptera: Cimicidae) from two southeastern bat caves, U.S.A. J Vector Ecol 2005; 30:339–341.
Renesto P, Gouvernet J, Drancourt M, Roux V, et al. Use of rpoB gene analysis for detection and identification of Bartonella species. J Clin Microbiol 2001; 39:430–437.
Veikkolainen V, Vesterinen EJ, Lilley TM, Pulliainen AT. Bats as reservoir hosts of human bacterial pathogen, Bartonella mayotimonensis. Emerg Infect Dis 2014; 20:960–967.
Information & Authors
Information
Published In
Vector-Borne and Zoonotic Diseases
Volume 17 • Issue Number 4 • April 2017
Pages: 243 - 246
PubMed: 28165925
Copyright
Copyright 2017, Mary Ann Liebert, Inc.
History
Published in print: April 2017
Published online: 1 April 2017
Published ahead of print: 6 February 2017
Topics
Authors
Author Disclosure Statement
No competing financial interests exist.
Metrics & Citations
Metrics
Citations
Export Citation
Export citation
Select the format you want to export the citations of this publication.
View Options
Access content
To read the fulltext, please use one of the options below to sign in or purchase access.⚠ Society Access
If you are a member of a society that has access to this content please log in via your society website and then return to this publication.