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Published Online: 17 September 2012

Methods for Culturing Saltwater Rotifers (Brachionus plicatilis) for Rearing Larval Zebrafish

Publication: Zebrafish
Volume 9, Issue Number 3

Abstract

The saltwater rotifer, Brachionus plicatilis, is widely used in the aquaculture industry as a prey item for first-feeding fishes due to its ease of culture, small size, rapid reproductive rate, and amenability to enrichment with nutrients. Despite the distinct advantages of this approach, rotifers have only been sporadically utilized for rearing larval zebrafish, primarily because of the common misconception that maintaining cultures of rotifers is difficult and excessively time-consuming. Here we present simple methods for maintaining continuous cultures of rotifers capable of supporting even the very largest zebrafish aquaculture facility, with minimal investments in materials, time, labor, and space. Examples of the methods' application in one large, existing facility is provided, and troubleshooting of common problems is discussed.

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References

1.
Cahu CInfante JZ. Substitution of live food by formulated diets in marine fish larvaeAquaculture2001200161-180. 1. Cahu C, Infante JZ. Substitution of live food by formulated diets in marine fish larvae. Aquaculture 2001;200:161–180.
2.
Hagiwara ASuga KAkazawa AKotani TSakakura Y. Development of rotifer strains with useful traits for rearing fish larvaeAquaculture200726844-52. 2. Hagiwara A, Suga K, Akazawa A, Kotani T, Sakakura Y. Development of rotifer strains with useful traits for rearing fish larvae. Aquaculture 2007;268:44–52.
3.
Westerfield MThe Zebrafish Book. A Guide for the Laboratory Use of Zebrafish4thUniversity of Oregon Press2007. 3. Westerfield M. The Zebrafish Book. A Guide for the Laboratory Use of Zebrafish. 4th ed: University of Oregon Press; 2007.
4.
Harper CLawrence CThe Laboratory ZebrafishBoca RatonCRC Press2010. 4. Harper C, Lawrence C. The Laboratory Zebrafish. Boca Raton: CRC Press; 2010.
5.
Lavens LSorgeloos PManual on the Production and Use of Live Food for AquacultureFAO Fisheries Technical Paper 361Rome1996. 5. Lavens L, Sorgeloos P. Manual on the Production and Use of Live Food for Aquaculture. FAO Fisheries Technical Paper 361, Rome, 1996.
6.
Carvalho APAraujo LSantos MM. Rearing zebrafish (Danio rerio) larvae without live food: Evaluation of a commercial, a practical and a purified starter diet on larval performanceAquaculture Res2006371107-1111. 6. Carvalho AP, Araujo L, Santos MM. Rearing zebrafish (Danio rerio) larvae without live food: Evaluation of a commercial, a practical and a purified starter diet on larval performance. Aquaculture Res 2006;37:1107–1111.
7.
Gómez-Requeni PConceição LOlderbakk Jordal ARønnestad I. A reference growth curve for nutritional experiments in zebrafish (Danio rerio) and changes in whole body proteome during developmentFish Physiol Biochem361199-12152010. 7. Gómez-Requeni P, Conceição L, Olderbakk Jordal A, Rønnestad I. A reference growth curve for nutritional experiments in zebrafish (Danio rerio) and changes in whole body proteome during development. Fish Physiol Biochem 36;1199–1215:2010.
8.
Hernández LPBarresi MJDevoto SH. Functional morphology and developmental biology of zebrafish: Reciprocal illumination from an unlikely coupleIntegr Comp Biol200242222-231. 8. Hernández LP, Barresi MJ, Devoto SH. Functional morphology and developmental biology of zebrafish: Reciprocal illumination from an unlikely couple. Integr Comp Biol 2002;42:222–231.
9.
Best JAdatto ICockington JJames ALawrence C. A novel method for rearing first-feeding larval zebrafish: Polyculture with Type L saltwater rotifers (Brachionus plicatilis)Zebrafish20107289-295. 9. Best J, Adatto I, Cockington J, James A, Lawrence C. A novel method for rearing first-feeding larval zebrafish: Polyculture with Type L saltwater rotifers (Brachionus plicatilis). Zebrafish 2010;7:289–295.
10.
Nusslein-Volhard CDahm RZebrafish, A Practical ApproachOxford University PressOxford2002. 10. Nusslein-Volhard C, Dahm R. Zebrafish, A Practical Approach. Oxford University Press, Oxford, 2002.
11.
Biga PGoetz F. Zebrafish and giant danio as models for muscle growth: Determinate vs. indeterminate growth as determined by morphometric analysisAm J Physiol Regul Integr Comp Physiol2006291R1327-1337. 11. Biga P, Goetz F. Zebrafish and giant danio as models for muscle growth: Determinate vs. indeterminate growth as determined by morphometric analysis. Am J Physiol Regul Integr Comp Physiol 2006;291:R1327–1337.
12.
Brown DD. The role of thyroid hormone in zebrafish and axolotl developmentProc Natl Acad Sci19979413011-13016. 12. Brown DD. The role of thyroid hormone in zebrafish and axolotl development. Proc Natl Acad Sci 1997;94:13011–13016.
13.
Lawrence CEnnis DGHarper CKent MLMurray KSanders GE. The challenges of implementing pathogen control strategies for fishes used in biomedical researchComp Biochem Physiol C Toxicol Pharmacol155160-1662012. 13. Lawrence C, Ennis DG, Harper C, Kent ML, Murray K, Sanders GE. The challenges of implementing pathogen control strategies for fishes used in biomedical research. Comp Biochem Physiol C Toxicol Pharmacol 155;160–166:2012.
14.
Hirayama KKusano T. Fundamental studies on physiology of rotifer for its mass culture. 2. Influence of water temperature on population-growth of rotiferBull Jap Soc Sci Fish1972381357-1363. 14. Hirayama K, Kusano T. Fundamental studies on physiology of rotifer for its mass culture. 2. Influence of water temperature on population-growth of rotifer. Bull Jap Soc Sci Fish 1972;38:1357–1363.
15.
Bentley CDCarroll PMWatanabe WORiedel AM. Intensive rotifer production in a pilot-scale continuous culture recirculating system using nonviable microalgae and an ammonia neutralizerJ World Aquacult Soc200839625-635. 15. Bentley CD, Carroll PM, Watanabe WO, Riedel AM. Intensive rotifer production in a pilot-scale continuous culture recirculating system using nonviable microalgae and an ammonia neutralizer. J World Aquacult Soc 2008;39:625–635.
16.
Hotos GN. Growth, filtration and ingestion rate of the rotifer Brachionus plicatilis fed with large (Asteromonas gracilis) and small (Chlorella sp.) celled algal speciesAquacult Res200334793-802. 16. Hotos GN. Growth, filtration and ingestion rate of the rotifer Brachionus plicatilis fed with large (Asteromonas gracilis) and small (Chlorella sp.) celled algal species. Aquacult Res 2003;34:793–802.
17.
Vadstein OOie GOlsen Y. Particle-size dependent feeding by the rotifer Brachionus-plicatilisHydrobiologia1993255261-267. 17. Vadstein O, Oie G, Olsen Y. Particle-size dependent feeding by the rotifer Brachionus-plicatilis. Hydrobiologia 1993;255:261–267.
18.
Lindemann NKleinow W. A study of rotifer feeding and digestive processes using erythrocytes as microparticulate markersHydrobiologia200043527-41. 18. Lindemann N, Kleinow W. A study of rotifer feeding and digestive processes using erythrocytes as microparticulate markers. Hydrobiologia 2000;435:27–41.
19.
Rainuzzo JRReitan KIOlsen Y. The significance of lipids at early stages of marine fish: A reviewAquaculture1997155103-115. 19. Rainuzzo JR, Reitan KI, Olsen Y. The significance of lipids at early stages of marine fish: A review. Aquaculture 1997;155:103–115.
20.
Rodriguez CPerez JAIzquierdo MSCejas JRBolanos ALorenzo A. Improvement of the nutritional value of rotifers by varying the type and concentration of oil and the enrichment periodAquaculture199614793-105. 20. Rodriguez C, Perez JA, Izquierdo MS, Cejas JR, Bolanos A, Lorenzo A. Improvement of the nutritional value of rotifers by varying the type and concentration of oil and the enrichment period. Aquaculture 1996;147:93–105.
21.
Lie OHaaland HHemre GIMaage ALied ERosenlund G et al. Nutritional composition of rotifers following a change in diet from yeast and emulsified oil to microalgaeAquacult Intl19975427-438. 21. Lie O, Haaland H, Hemre GI, Maage A, Lied E, Rosenlund G, et al. Nutritional composition of rotifers following a change in diet from yeast and emulsified oil to microalgae. Aquacult Intl 1997;5:427–438.
22.
Korstad JVadstein OOlsen Y. Feeding kinetics of Brachionus plicatilis fed Isochrysis galbanaHydrobiologia198918651-57. 22. Korstad J, Vadstein O, Olsen Y. Feeding kinetics of Brachionus plicatilis fed Isochrysis galbana. Hydrobiologia 1989;186:51–57.
23.
Battaglene SCCobcroft JM. Advances in the culture of striped trumpeter larvae: A reviewAquaculture2007268195-208. 23. Battaglene SC, Cobcroft JM. Advances in the culture of striped trumpeter larvae: A review. Aquaculture 2007;268:195–208.
24.
Cobcroft JShu-Chien AKuah M-KJaya-Ram ABattaglene S. The effects of tank color, live food enrichment and green water on the early insert of jaw malformation in striped trumpeter larvaeAquaculture2012356–35761-72. 24. Cobcroft J, Shu-Chien A, Kuah M-K, Jaya-Ram A, Battaglene S. The effects of tank color, live food enrichment and green water on the early insert of jaw malformation in striped trumpeter larvae. Aquaculture 2012;356–357:61–72.
25.
Reitan KIRainuzzo JROie GOlsen Y. A review of the nutritional effects of algae in marine fish larvaeAquaculture1997155207-221. 25. Reitan KI, Rainuzzo JR, Oie G, Olsen Y. A review of the nutritional effects of algae in marine fish larvae. Aquaculture 1997;155:207–221.
26.
van der Meeren TMangor-Jensen APickova J. The effect of green water and light intensity on survival, growth and lipid composition in Atlantic cod (Gadus morhua) during intensive larval rearingAquaculture2007265206-217. 26. van der Meeren T, Mangor-Jensen A, Pickova J. The effect of green water and light intensity on survival, growth and lipid composition in Atlantic cod (Gadus morhua) during intensive larval rearing. Aquaculture 2007;265:206–217.
27.
Yamasaki SSecor DHHirata H. Population-growth of 2 types of rotifer (l and s) Brachionus plicatilis at different dissolved-oxygen levelsNippon Suisan Gakkaishi1987531303. 27. Yamasaki S, Secor DH, Hirata H. Population-growth of 2 types of rotifer (l and s) Brachionus plicatilis at different dissolved-oxygen levels. Nippon Suisan Gakkaishi 1987;53:1303.
28.
Oie GOlsen Y. Influence of rapid changes in salinity and temperature on the mobility of the rotifer Brachionus plicatilisHydrobiologia.199325581-86. 28. Oie G, Olsen Y. Influence of rapid changes in salinity and temperature on the mobility of the rotifer Brachionus plicatilis. Hydrobiologia. 1993;255:81–86.
29.
Epp RWWinston PW. Effects of salinity and ph on activity and oxygen-consumption of Brachionus plicatilis (Rotatoria)Comp Biochem Physiol1978599-12. 29. Epp RW, Winston PW. Effects of salinity and ph on activity and oxygen-consumption of Brachionus plicatilis (Rotatoria). Comp Biochem Physiol 1978;59:9–12.
30.
Joshi P. Influence of salinity on population growth of a rotifer, Brachionus plicatilis, (Mullen)J Indian Fish Assoc19881875-81. 30. Joshi P. Influence of salinity on population growth of a rotifer, Brachionus plicatilis, (Mullen). J Indian Fish Assoc 1988;18:75–81.
31.
Jellison RAdams HMelack JM. Re-appearance of rotifers in hypersaline Mono Lake, California, during a period of rising lake levels and decreasing salinityHydrobiologia200146639-43. 31. Jellison R, Adams H, Melack JM. Re-appearance of rotifers in hypersaline Mono Lake, California, during a period of rising lake levels and decreasing salinity. Hydrobiologia 2001;466:39–43.
32.
Minkoff GLubzens EMeragelman E. Improving asexual reproduction rates in a rotifer (Brachionus plicatilis) by salinity manipulationsIsr J Zool198533195-203. 32. Minkoff G, Lubzens E, Meragelman E. Improving asexual reproduction rates in a rotifer (Brachionus plicatilis) by salinity manipulations. Isr J Zool 1985;33:195–203.
33.
Lubzens EMinkoff GMarom S. Salinity dependence of sexual and asexual reproduction in the rotifer Brachionus plicatilisMarine Biol198585123-126. 33. Lubzens E, Minkoff G, Marom S. Salinity dependence of sexual and asexual reproduction in the rotifer Brachionus plicatilis. Marine Biol 1985;85:123–126.
34.
Cheng SHAoki SMaeda MHino A. Competition between the rotifer Brachionus rotundiformis and the ciliate Euplotes vannus fed on two different algaeAquaculture2004241331-343. 34. Cheng SH, Aoki S, Maeda M, Hino A. Competition between the rotifer Brachionus rotundiformis and the ciliate Euplotes vannus fed on two different algae. Aquaculture 2004;241:331–343.
35.
Yu JPHirayama K. Study on the unexpected sudden decrease and suppressed growth of the rotifer population in mass-culture. 1. The effect of un-ionized ammonia on the population-growth of the rotifer in mass-cultureBull Jap Soc SciFish1986521509-1513. 35. Yu JP, Hirayama K. Study on the unexpected sudden decrease and suppressed growth of the rotifer population in mass-culture. 1. The effect of un-ionized ammonia on the population-growth of the rotifer in mass-culture. Bull Jap Soc SciFish 1986;52:1509–1513.

Information & Authors

Information

Published In

cover image Zebrafish
Zebrafish
Volume 9Issue Number 3September 2012
Pages: 140 - 146
PubMed: 22950820

History

Published online: 17 September 2012
Published ahead of print: 5 September 2012
Published in print: September 2012

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Affiliations

Christian Lawrence
Aquatic Resources Program, Children's Hospital Boston, Boston, Massachusetts.
Erik Sanders
Department of Development Biology, Washington University School of Medicine, St. Louis, Missouri.
Eric Henry
Reed Mariculture, Inc., Campbell, California.

Notes

Address correspondence to:Christian Lawrence, M.S.Aquatic Resources ProgramChildren's Hospital Boston320 Longwood AvenueBoston, MA 02115E-mail: [email protected]

Disclosure Statement

Eric Henry is employed by Reed Mariculture, Inc.

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