Research Article
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Published Online: 28 June 2016

RESAMA: A Network for Monitoring Health and Husbandry Practices in Aquatic Research Facilities

Publication: Zebrafish
Volume 13, Issue Number S1


Health monitoring is a crucial aspect of the management of any research animal house. RESAMA is a network strong of 60 academic and private partners acting in France since the end of 2012. The network aims to increase awareness of animal caretakers and researchers on health management issues in facilities holding aquatic model species (zebrafish, Xenopus, medaka, Mexican tetra). To do so, each partner research facility will be visited at least once. The visiting team is composed at least of one veterinarian and one zootechnician specialized in aquatic species. The visit results in a health-monitoring assessment of the facility, which includes a sampling for histo-pathological, bacteriological, and molecular pathogen detection. During the visit, rearing practices are also reviewed through an interview of animal caretakers. However, the present report essentially focuses on the health-monitoring aspect. The ultimate goal of the project is to provide a network-wide picture of health issues in aquatic facilities. Performed in parallel, the rearing practice assessment will ultimately help to establish rational relationship between handling practices and animal health in aquatic facilities. The study is still in progress. Here, we describe the results to be drawn from an analysis of the 23 facilities that had been visited so far. We sampled 720 fish and 127 amphibians and performed a little less than 1400 individual tests.

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Published In

cover image Zebrafish
Volume 13Issue Number S1July 2016
Pages: S-56 - S-65
PubMed: 27192449


Published in print: July 2016
Published online: 28 June 2016
Published ahead of print: 18 May 2016


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    Laurent Legendre
    UMS AMAGEN, CNRS, INRA, Université Paris-Saclay, Gif sur Yvette, France.
    Brigitte Guillet
    CRB Xénope, UMS 3387 CNRS, Université de Rennes 1, Rennes, France.
    Emmanuel Leguay
    VETOFISH, Châteauneuf-les-Martigues, France.
    Emmanuel Meunier
    VETOFISH, Châteauneuf-les-Martigues, France.
    Sophie Labrut
    Université LUNAM, ONIRIS, Nantes, France.
    Nicolas Keck
    Laboratoire Départemental Vétérinaire de l'Hérault, Montpellier, France.
    Massimiliano Bardotti
    UMR 9197 Neuro-PSI, CNRS, Université Paris-Sud, Université Paris-Saclay, Gif sur Yvette, France.
    Lorraine Michelet
    Université Paris-Est, Laboratoire National de Référence de la Tuberculose, Unité de Zoonoses Bactériennes, Laboratoire de Santé Animale, ANSES, Maisons-Alfort, France.
    Frédéric Sohm
    UMS AMAGEN, CNRS, INRA, Université Paris-Saclay, Gif sur Yvette, France.


    Address correspondence to:Laurent Legendre, BScUMS AMAGEN, CNRS, INRACNRS, bâtiment 32, 1 Avenue de la terrasseGif sur Yvette 91190France
    E-mail: [email protected]

    Author Contributions

    B.G., L.L., and E.L. participated equally in this work. B.G., L.L., E.L., and F.S. designed the concept; E.M. has designed and written the database implementation and the website; B.G., L.L., E.L., and F.S. participated in the visits; E.L. performed necropsy with the help of B.G. and L.L.; L.M. and N.K. carried out the PCR and sequencing analysis; bacteriology was performed by N.K.; S.L. performed anatomo-pathology in coordination with E.L. All the authors participated in the analysis of the results; F.S. and E.L. wrote the article with the help of all the authors.

    Disclosure Statement

    No competing financial interests exist.

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