Research Article
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Published Online: 28 June 2016

Effects of Subclinical Mycobacterium chelonae Infections on Fecundity and Embryo Survival in Zebrafish

Publication: Zebrafish
Volume 13, Issue Number S1

Abstract

Mycobacteriosis is the second most common infectious disease in zebrafish research colonies, and most often this is caused by Mycobacterium chelonae. The infection is characterized by multiple granulomas in the kidney, coelomic cavity, particularly the ovary. However, most fish still appear clinically normal. Developmental genetics remain a primary area of research with the zebrafish model, and hence, an important use of adult zebrafish is as brood fish to produce embryos. We investigated the effects of experimentally induced M. chelonae infections on fecundity. A total of 480 5D wild-type zebrafish were divided into four groups: controls, males infected, females infected, and both sexes. Exposed fish developed high prevalence of infection, including many females with ovarian infections. Fish were then first subjected to four separate group spawns with four replicate tanks/group. Then, a third of the fish were subjected to pairwise spawns, representing 20 pairs/group, and then the pairs were evaluated by histopathology. Overall, the group and pairwise spawns resulted numerous eggs and viable embryos. However, we found no statistical correlations between infection status and number of eggs or viability. In contrast to Egg Associated Inflammation and Fibroplasia, lesions in infected ovaries were more localized, with large regions of the ovary appearing normal.

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References

1.
Kent ML, Whipps CM, Matthews JL, Florio D, Watral V, Bishop-Stewart JK, et al. Mycobacteriosis in zebrafish (Danio rerio) research facilities. Comp Biochem Physiol Part C 2004;138:383–390.
2.
Murray KN, Bauer J, Tallen A, Matthews JL, Westerfield M, Varga ZM. Characterization and management of asymptomatic Mycobacterium infections at the Zebrafish International Resource Center. J Am Assoc Lab Anim Sci 2011;50:675–679.
3.
Whipps CM, Matthews JL, Kent ML. Distribution and genetic characterization of Mycobacterium chelonae in laboratory zebrafish Danio rerio. Dis Aquatic Org 2008;82:45–54.
4.
Whipps CM, Lieggi C, Wagner R. Mycobacteriosis in zebrafish colonies. ILAR J 2012;53:95–105.
5.
Astrofsky KM, Schrenzel MD, Bullis RA, Smolowitz RM, Fox JG. Diagnosis and management of atypical Mycobacterium spp. infections in established laboratory zebrafish (Brachydanio rerio) facilities. Comp Med 2000;50:666–672.
6.
Watral V, Kent ML. Pathogenesis of Mycobacterium spp. in zebrafish (Danio rerio) from research facilities. Comp Biochem Physiol Part C 2007;145:55–60.
7.
Ostland VE, Watral V, Whipps CM, Austin FW, St-Hilaire S, Westerman ME,et al. Biochemical, molecular, and virulence characteristics of select Mycobacterium marinum isolates in hybrid striped bass (Morone chrysops x Morone saxatilis) and zebrafish (Danio rerio). Dis Aquat Org 2008;79:107–118.
8.
Whipps CM, Dougan ST, Kent ML. Mycobacterium haemophilum infections of zebrafish (Danio rerio) in research facilities. FEMS Microbiol Lett 2007;270:21–26.
9.
Kent ML, Harper C, Wolf JC. Documented and potential research impacts of subclinical diseases in zebrafish. ILAR J 2012;53:126–134.
10.
Miller GW, Truong L, Barton CL, Labut EM, Bebold KM, Traber MG, et al. The influences of parental diet and vitamin E intake on the embryonic zebrafish transcriptome. Comp Biochem Physiol D Genom Proteom 2014;10:22–29.
11.
Peterson TS, Kent ML, Ferguson JA, Watral VG, Whipps CM. Comparison of fixatives and fixation time on PCR detection of Mycobacterium in zebrafish Danio rerio. Dis Aquat Org 2013;104:113–120.
12.
Spence R, Gerlach G. Lawrence C, Smith C. The behavior and ecology of the zebrafish, Danio rerio. Biol Rev 2008:83:13–34.
13.
Castranova D, Lawton A, Lawrence C, Baumann DP, Best J, Coscolla J, et al. The effect of stocking densities on reproductive performance in laboratory zebrafish (Danio rerio). Zebrafish 2011;8:141–146.
14.
Engeszer RE, Patterson LB, Rao AA, Parichy DM. Zebrafish in the wild: A review of natural history and new notes from the field. Zebrafish 2007;4:21–40.
15.
Harper C, Lawernce C: The Laboratory Zebrafish. CRC Press, Boca Raton, FL and Smith, 2011.
16.
Ramsay JM, Watral V, Schreck CB, Kent ML. Husbandry stress exacerbates mycobacterial infections in adult zebrafish, Danio rerio (Hamilton). J Fish Dis 2009;32:931–941.
17.
Kent ML, Buchner C, Watral VG, Sanders JL, LaDu J, Peterson TS, et al. Development and maintenance of a specific pathogen-free (SPF) zebrafish research facility for Pseudoloma neurophilia. Dis Aquat Org 2011;95:73–79.
18.
Rossteuscher S, Schmidt-Posthaus H, Schäfers C, Teigeler T, Segner H. Background pathology of the ovary in a laboratory population of zebrafish Danio rerio. Dis Aquat Org 2008;79:169–172.
19.
Ariëns Kappers CU. The meninges in Cyclostomes, Selachians, and Teleosts, compared with those in man. Proc R Acad Amst 1924;28:71–80.
20.
Caruncho HJ, Da Silva PP. Alterations in the intermediate layer of goldfish meninges during adaptation to darkness. J Anat 1994:184:355–362.

Information & Authors

Information

Published In

cover image Zebrafish
Zebrafish
Volume 13Issue Number S1July 2016
Pages: S-88 - S-95
PubMed: 27031171

History

Published in print: July 2016
Published online: 28 June 2016
Published ahead of print: 31 March 2016

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Authors

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Michael L. Kent
Department of Microbiology, Oregon State University, Corvallis, Oregon.
Department of Biomedical Sciences, Oregon State University, Corvallis, Oregon.
Virginia G. Watral
Department of Microbiology, Oregon State University, Corvallis, Oregon.
Nicole S. Kirchoff
Department of Microbiology, Oregon State University, Corvallis, Oregon.
Sean T. Spagnoli
Department of Biomedical Sciences, Oregon State University, Corvallis, Oregon.
Thomas J. Sharpton
Department of Microbiology, Oregon State University, Corvallis, Oregon.
Department of Statistics, Oregon State University, Corvallis, Oregon.

Notes

Address correspondence to:Michael L. Kent, PhDDepartment of MicrobiologyOregon State University226 Nash HallCorvallis, OR 97331E-mail: [email protected]

Disclosure Statement

No competing financial interests exist.

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