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Published Online: 10 September 2015

Houttuynia cordata Extract Improves Physical Endurance Performance by Regulating Endothelial Production of Nitric Oxide

Publication: Journal of Medicinal Food
Volume 18, Issue Number 9


Vascular function is mediated by various regulatory molecules, including endothelial nitric oxide (NO), which regulates the vasodilation of smooth muscle cells. We investigated whether standardized Houttuynia cordata extract (SHCE) could improve physical endurance performance by regulating the endothelial production of NO. For the standardization of Houttuynia cordata (HC) extract, its bioactive components were identified and quantified using ultraperformance liquid chromatography–mass spectrometry. Bioaccessibility and biological activity were measured by the in vitro digestion model system and free radical scavenging capacity, respectively. The vascular function in the endothelium was assessed by the phosphorylation of endothelial nitric oxide synthase (eNOS). A preliminary clinical trial was carried out to assess the physical endurance performance. HC extract was standardized to bioactive components, including chlorogenic acid, rutin, and quercitrin, with the concentration of 5.53, 6.09, and 16.15 mg from 1 g of dry weight, respectively. Bioaccessibility was 33.17%, 31.67%, and 11.18% for chlorogenic acid, rutin, and quercitrin, respectively. Antioxidant activities of SHCE were expressed as vitamin C equivalent antioxidant capacity in 55.81 and 17.23 mg/g of HC extract using ABTS and DPPH scavenging assay, respectively. In human aortic endothelial cells, insulin-mediated phosphorylation of eNOS was increased by SHCE in the presence of palmitate. However, the expression of blood pressure-regulating genes was not altered. The level of blood lactate concentration and the heart rate of subjects who drank SHCE were lower than those of subjects who drank plain water. Oxygen uptake from subjects drinking SHCE was slightly higher than that from those who drank plain water. This study demonstrated that SHCE decreased heart rate and blood lactate, increased oxygen uptake, and improved physical performance, presumably due to the increased NO production.

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Published In

cover image Journal of Medicinal Food
Journal of Medicinal Food
Volume 18Issue Number 9September 2015
Pages: 1022 - 1031
PubMed: 25923355


Published online: 10 September 2015
Published in print: September 2015
Published ahead of print: 29 April 2015
Accepted: 23 March 2015
Received: 4 November 2014


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Ui-Jeong Yang
Department of Food Science and Technology, Sejong University, Seoul, Korea.
Hyojin Maeng
Department of Life Science, Gachon University, Gyeonggi, Korea.
Tae-Sik Park
Department of Life Science, Gachon University, Gyeonggi, Korea.
Soon-Mi Shim
Department of Food Science and Technology, Sejong University, Seoul, Korea.


Address correspondence to: Tae-Sik Park, PhD, Department of Life Science, Gachon University, Gyeonggi 461-701, Republic of Korea, E-mail: [email protected] or Soon-Mi Shim, PhD, Department of Food Science and Technology, Sejong University, Seoul 143-747, Republic of Korea, E-mail: [email protected]

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No competing financial interests exist.

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