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Published Online: 13 April 2016

Extracts from Lentinula edodes (Shiitake) Edible Mushrooms Enriched with Vitamin D Exert an Anti-Inflammatory Hepatoprotective Effect

Publication: Journal of Medicinal Food
Volume 19, Issue Number 4


Vitamin D has been known for its anti-inflammatory properties. Extracts derived from Lentinula edodes (Shiitake) edible mushroom exert an anti-inflammatory effect. These extracts contain high levels of ergosterol, which converts into ergocalciferol (vitamin D2) following exposure to ultraviolet light, followed by absorption and hydroxylation into the active form 25-hydroxyvitamin D [25(OH)D]. To determine the anti-inflammatory effect of overexpression of vitamin D in edible mushrooms, L. edodes mushrooms were exposed to ultraviolet-B light, freeze-dried, followed by measurement of vitamin D2 contents, in their dry weight. C57B1/6 mice were orally treated with vitamin D2-enriched or nonenriched mushroom extract prior and during concanavalin A-immune-mediated liver injury. Exposure to ultraviolet light increased vitamin D2 content in Shiitake edible mushrooms. Following feeding of vitamin D-enriched mushroom extracts to mice with immune-mediated hepatitis, a significant decrease in liver damage was noted. This was shown by a decrease in alanine aminotransferase and aspartate aminotransferase serum levels, a decrease in proportion of mice with severe liver injury, and by improvement in liver histology. These effects were associated with a decrease in serum interferon gamma levels. A synergistic effect was noted between the anti-inflammatory effect of the mushroom extracts and that of vitamin D. Oral administration of vitamin D-enriched L. edodes edible mushroom exerts a synergistic anti-inflammatory effect in the immune-mediated hepatitis. The data support its potential use as safe immunomodulatory adjuvant for the treatment of HCV and nonalcoholic steatohepatitis.

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Published In

cover image Journal of Medicinal Food
Journal of Medicinal Food
Volume 19Issue Number 4April 2016
Pages: 383 - 389
PubMed: 27027234


Published online: 13 April 2016
Published in print: April 2016
Published ahead of print: 30 March 2016
Accepted: 17 January 2016
Received: 1 October 2015


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Ariel Drori
Liver Unit, Department of Medicine, Hadassah - Hebrew University Medical Center, Jerusalem, Israel.
Yehudit Shabat
Liver Unit, Department of Medicine, Hadassah - Hebrew University Medical Center, Jerusalem, Israel.
Ami Ben Ya'acov
Liver Unit, Department of Medicine, Hadassah - Hebrew University Medical Center, Jerusalem, Israel.
Ofer Danay
Migal, Galilee Research Institute, Kiryat Shmone, Israel.
Dan Levanon
Migal, Galilee Research Institute, Kiryat Shmone, Israel.
Lidya Zolotarov
Liver Unit, Department of Medicine, Hadassah - Hebrew University Medical Center, Jerusalem, Israel.
Yaron Ilan
Liver Unit, Department of Medicine, Hadassah - Hebrew University Medical Center, Jerusalem, Israel.


Address correspondence to: Yaron Ilan, MD, Liver Unit, Department of Medicine, Hadassah-Hebrew University Medical Center, P.O.B 12000, Jerusalem IL-91120, Israel, E-mail: [email protected]

Author Disclosure Statement

No competing financial interests exist.

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